Overview

  • Product name
    Anti-Histone H1.0 antibody
    See all Histone H1.0 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.0
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Histone H1.0 aa 1-194. The exact sequence is proprietary.
    Database link: P07305

  • Positive control
    • HepG2 whole cell lysate; HepG2 nuclear lysate; U20S cells; SkHep1 xenograft; C2C12 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab154111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
  • Sequence similarities
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-17 in RNA edited version.
  • Cellular localization
    Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member 0 antibody
    • H1(0) antibody
    • H10 antibody
    • H10_HUMAN antibody
    • h1f0 antibody
    • H1FV antibody
    • Histone H1'' antibody
    • Histone H1(0) antibody
    • Histone H1.0 antibody
    • Histone H10 antibody
    • Histone H5 antibody
    • MGC5241 antibody
    • N-terminally processed antibody
    see all

Images

  • All lanes : Anti-Histone H1.0 antibody (ab154111) at 1/5000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : HepG2 nuclear lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 20 kDa

  • Immunofluorescent analysis of paraformaldehyde fixed U20S cells labeling Histone H1 with ab154111 (green) at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/2000.
  • Immunohistochemical analysis of paraffin embedded SkHep1 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin embedded C2C12 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.

References

ab154111 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154111.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up