Overview

  • Product nameAnti-Histone H1.0 antibody
    See all Histone H1.0 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.0
  • SpecificityDetects endogenous levels of total Histone 1.0 protein
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H1.0 aa 71-120 (internal sequence).
    Database link: P07305

  • Positive control
    • A549 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesaffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA 1/40000.

Target

  • FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-17 in RNA edited version.
  • Cellular localizationNucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member 0 antibody
    • H1(0) antibody
    • H10 antibody
    • H10_HUMAN antibody
    • h1f0 antibody
    • H1FV antibody
    • Histone H1'' antibody
    • Histone H1(0) antibody
    • Histone H1.0 antibody
    • Histone H10 antibody
    • Histone H5 antibody
    • MGC5241 antibody
    • N-terminally processed antibody
    see all

Anti-Histone H1.0 antibody images

  • All lanes : Anti-Histone H1.0 antibody (ab65000) at 1/500 dilution

    Lane 1 : Extracts from A549 cells at 10-30µg
    Lane 2 : Extracts from A549 cells at 10-30µg with immunising peptide at 5-10µg


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 110 kDa,23 kDa,32 kDa,34 kDa,36 kDa,46 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Histone H1.0 antibody (ab65000)

ab65000 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"