Overview

  • Product nameAnti-Histone H1.0 antibody [EPR6537]See all Histone H1.0 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR6537] to Histone H1.0
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H1.0 aa 150 to the C-terminus (C terminal).
    Database link: P07305

  • Positive control
    • WB: BxPC3 and fetal Human lung lysates IHC-P: Human kidney tissue ICC/IF: A431 cells
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab125027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
    • Sequence similaritiesBelongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-17 in RNA edited version.
    • Cellular localizationNucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • dJ221C16.2 antibody
      • dJ221C16.5 antibody
      • DmeH1 antibody
      • H1 0 histone antibody
      • H1 antibody
      • H1 histone family member 0 antibody
      • H1 histone family member 1 antibody
      • H1 histone family member 3 antibody
      • H1 histone family member 5 antibody
      • H1 histone family member N testis specific antibody
      • H1 histone family member O oocyte specific antibody
      • H1 histone family member X antibody
      • H1(0) antibody
      • H1.1 antibody
      • H1.2 antibody
      • H1.3 antibody
      • H1.4 antibody
      • H1.5 antibody
      • H10 antibody
      • H1A antibody
      • H1f0 antibody
      • H1F0 protein antibody
      • H1F1 antibody
      • H1F2 antibody
      • H1F3 antibody
      • H1F4 antibody
      • H1F5 antibody
      • H1FNT antibody
      • H1FOO antibody
      • H1FOO protein antibody
      • H1FOO_HUMAN antibody
      • H1FT antibody
      • H1FV antibody
      • H1FX antibody
      • H1oo antibody
      • H1t antibody
      • H1T2 antibody
      • H1X antibody
      • HANP1 antibody
      • His1 antibody
      • HisC antibody
      • HIST1 antibody
      • HIST1H1A antibody
      • HIST1H1B antibody
      • HIST1H1C antibody
      • HIST1H1D antibody
      • HIST1H1E antibody
      • HIST1H1T antibody
      • Histone 1 H1a antibody
      • Histone 1 H1b antibody
      • Histone 1 H1c antibody
      • Histone 1 H1d antibody
      • Histone 1 H1e antibody
      • Histone 1 H1t antibody
      • Histone antibody
      • Histone H1.0 antibody
      • Histone H1.1 antibody
      • Histone H1.2 antibody
      • Histone H1.3 antibody
      • Histone H1.4 antibody
      • Histone H1.5 antibody
      • Histone H1oo antibody
      • Histone H1x antibody
      • MGC116819 antibody
      • MGC126630 antibody
      • MGC126632 antibody
      • MGC126642 antibody
      • MGC138176 antibody
      • MGC138345 antibody
      • MGC15959 antibody
      • MGC3992 antibody
      • MGC50807 antibody
      • MGC5241 antibody
      • MGC8350 antibody
      • Oocyte specific histone H1 antibody
      • Oocyte specific linker histone H1 antibody
      • Oocyte-specific histone H1 antibody
      • Oocyte-specific linker histone H1 antibody
      • osH1 antibody
      • Testicular H1 histone antibody
      see all

    Anti-Histone H1.0 antibody [EPR6537] images

    • All lanes : Anti-Histone H1.0 antibody [EPR6537] (ab125027) at 1/1000 dilution

      Lane 1 : BxPC3 lysates
      Lane 2 : Human fetal lung lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 21 kDa
    • ab125027, at a 1/100 dilution, staining Histone H1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab125027, at a 1/100 dilution, staining Histone H1 in A431 cells by Immunofluorescence.

    References for Anti-Histone H1.0 antibody [EPR6537] (ab125027)

    This product has been referenced in:
    • Kong X  et al. GSK3ß is a checkpoint for TNF-a-mediated impaired osteogenic differentiation of mesenchymal stem cells in inflammatory microenvironments. Biochim Biophys Acta 1830:5119-29 (2013). Read more (PubMed: 23911749) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    H1.2 specific antibodies,
    ab4086; http://www.abcam.com/Histone-H1-2-antibody-ChIP-Grade-ab4086.html. (we however haven't tested the specificity to other forms)
    ab17677; http://www.abcam.com/Histone-H1-2-...

    Read More

    Thank you for contacting us.

    ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

    ab83058; the sequence used is from P07305 http://...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"