Overview

  • Product name
    Anti-Histone H1.01 antibody
  • Description
    Sheep polyclonal to Histone H1.01
  • Tested applications
    Suitable for: WB, IHC-P, ChIPmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    N-terminal peptide (

    APAAAPDAPAPGAK-C

    ) of chicken linker histone H1.01.

  • Positive control
    • IHC-P: Chicken normal colon FFPE tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.

Images

  • IHC image of ab100944 staining Histone H1.01 in chicken colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab100944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Competition Western Blot Analysis. The recombinant linker histone was separated on a 15% SDS PAGE slab gel together with a track loaded with total chicken histone as marker and transferred to nitrocellulose membranes. These were then cut into strips of equal width and each strip developed with the antibody which had been pre-incubated with one of the recombinant linker histone at four increasing concentrations (two are shown). The panels show (from left to right): the transfer of the recombinant protein slab gel; the transfer of a total chicken histone marker, M; the histone marker strip blotted with the antibody under test, M(WB), showing that there is no recognition of the core histones, nor H5, with the antibody; six strips developed with the test antibody (at 0.1 mg/ml) pre-incubated with the six recombinant histones (01 to R) at the indicated concentrations. The Control lanes represent blots of a seventh strip developed with the test antibody without any competitor. The red boxes

References

This product has been referenced in:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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