Overview

  • Product name
    Anti-Histone H1.10 antibody
  • Description
    Sheep polyclonal to Histone H1.10
  • Tested applications
    Suitable for: WB, ChIP, IHC-Pmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    N-terminal peptide (APAAAPAVAAPAAK-C) of chicken linker histone H1.01.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Images

  • Competition Western Blot Analysis. The recombinant linker histone was separated on a 15% SDS PAGE slab gel together with a track loaded with total chicken histone as marker and transferred to nitrocellulose membranes. These were then cut into strips of equal width and each strip developed with the antibody which had been pre-incubated with one of the recombinant linker histone at four increasing concentrations (two are shown). The panels show (from left to right): the transfer of the recombinant protein slab gel; the transfer of a total chicken histone marker, M; the histone marker strip blotted with the antibody under test, M(WB), showing that there is no recognition of the core histones, nor H5, with the antibody; six strips developed with the test antibody (at 0.1 mg/ml) pre-incubated with the six recombinant histones (01 to R) at the indicated concentrations. The Control lanes represent blots of a seventh strip developed with the test antibody without any competitor. The red boxes show tracks in which signal has been competed away.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

We have not tested the H1.10 antibody ab100947 against the human or mouse proteins, but I ran a BLAST of the immunogen to these species and there was no significant similarity detected, so we would not predict the antibody to react. The gallus H...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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