Anti-Histone H1.3 antibody (ab24174)
Key features and details
- Rabbit polyclonal to Histone H1.3
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H1.3 antibody
See all Histone H1.3 primary antibodies -
Description
Rabbit polyclonal to Histone H1.3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide:
LAPTIPAPAEKTPVKKKAKKAGATAGK -C
, corresponding to N terminal amino acids 7-33 of Human Histone H1.3 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 20% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa). H1.3 is present in many human cell lines. Cross-reacts with bands of approx. 52kDa and 45kDa in nuclear extracts of T47D cell line.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50. |
WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa). H1.3 is present in many human cell lines. Cross-reacts with bands of approx. 52kDa and 45kDa in nuclear extracts of T47D cell line. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3007 Human
- GenBank: NP_005320 Human
- Omim: 142210 Human
- SwissProt: P16402 Human
- Unigene: 136857 Human
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Alternative names
- H1 histone family member 3 antibody
- H1.3 antibody
- H13_HUMAN antibody
see all
Images
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Rabbit polyclonal to Histone H1.3 against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line. Notice that extra bands are present/detected in the chromatin preparation.
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Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab24174 (1/200) for Histone H1.3 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.
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IHC image of ab24174 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24174, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab24174 has been referenced in 13 publications.
- Serna-Pujol N et al. Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion. Nucleic Acids Res 50:3892-3910 (2022). PubMed: 35380694
- Garciaz S et al. Epigenetic down-regulation of the HIST1 locus predicts better prognosis in acute myeloid leukemia with NPM1 mutation. Clin Epigenetics 11:141 (2019). PubMed: 31606046
- Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res 28:756-770 (2018). PubMed: 29844578
- Izquierdo-Bouldstridge A et al. Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats. Nucleic Acids Res 45:11622-11642 (2017). PubMed: 28977426
- Mayor R et al. Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions. J Biol Chem 290:7474-91 (2015). WB ; Human . PubMed: 25645921