Overview

  • Product name
    Anti-Histone H1.4 antibody
    See all Histone H1.4 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.4
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, African green monkey
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MSETAPAAPA APAPAEKTPV KKKARKSAGA AKRKASGPPV SELITKAVAA) of Human Histone H1.4 (NP_005312).

  • Positive control
    • ACHN cell lysate IF/ICC: SKNSH cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.Can be blocked with Histone H1.4 peptide (ab130902). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    modifications
    Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ221C16.5 antibody
    • H1 histone family, member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1, H1e antibody
    • Histone cluster 1, H1e antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    • OTTHUMP00000016139 antibody
    see all

Images

  • ICC/IF image of ab105522 stained SKNSH cells. The cells were4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-Histone H1.4 antibody (ab105522) at 1 µg/ml + ACHN cell lysate at 10 µg

    Predicted band size : 22 kDa
    Gel concentration: 12%

References

ab105522 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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