• Product nameAnti-Histone H1.4 antibody
    See all Histone H1.4 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.4
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 1-30 of Human Histone H1.4 (NP_005312.1).

  • Positive control
    • MDA-MB435 cell line lysates. IHC-P: Human normal colon FFPE tissue sections.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab116393 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab116393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 22 kDa.


  • FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ221C16.5 antibody
    • H1 histone family, member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1, H1e antibody
    • Histone cluster 1, H1e antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    • OTTHUMP00000016139 antibody
    see all

Anti-Histone H1.4 antibody images

  • IHC image of ab116393 staining Histone H1.4 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116393, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Histone H1.4 antibody (ab116393) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 22 kDa

References for Anti-Histone H1.4 antibody (ab116393)

ab116393 has not yet been referenced specifically in any publications.

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