Anti-Histone H1.4 (phospho T18) antibody (ab3595)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 32 kDa.
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    modifications
    Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1 H1e antibody
    • Histone cluster 1 H1e antibody
    • Histone H1 antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    see all

Anti-Histone H1.4 (phospho T18) antibody images

  • Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).

    Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.

    Lanes 1-2    :  wt Histone H1
    Lanes 3-4    :  T146A, T154A, S172A, S187A (wt site at T18)
    Lanes 5-6    :  T18A, T154A, S172A, S187A (wt site at T146)
    Lanes 7-8    :  T18A, T146A, S172A, S187A (wt site at T154)
    Lanes 9-10  :  T18A, T146A, T154A, S187A (wt site at S172)
    Lanes 11-12:  T18A, T146A, T154A, S172A (wt site at S187)
    Lanes 13-14:  T18A, T146A, T154A, S172A, S187A

    Alejandro Contreras, Baylor College of Medicine

  • Rabbit polyclonal to Histone H1 (phospho T18) (1/25).

    Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab3595 (green). The DNA stained with DAPI is shown in red. (100x magnification).

References for Anti-Histone H1.4 (phospho T18) antibody (ab3595)

This product has been referenced in:
  • Chen Y  et al. Quantitative Mass Spectrometry Reveals that Intact Histone H1 Phosphorylations are Variant Specific and Exhibit Single Molecule Hierarchical Dependence. Mol Cell Proteomics N/A:N/A (2015). Read more (PubMed: 26209608) »

See 1 Publication for this product

Product Wall

Thank you for your email and I'm sorry to hear that you are experiencing difficulty with ab3595. It would be very helpful if you could provide me with some additional details regarding your protocol. This will help us to investigate this matter as quic...

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Thank you for your e-mail. We are very sorry to hear that you are having problem with this antibody. We do not have problem with this antibody and this is the first complaint we have received so far. Certainly, we can send you a new vial as a repl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"