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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1, phosphorylated at T18.
(Peptide available as ab10138.)
Our Abpromise guarantee covers the use of ab3595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 32 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).
Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.
Lanes 1-2 : wt Histone H1
Lanes 3-4 : T146A, T154A, S172A, S187A (wt site at T18)
Lanes 5-6 : T18A, T154A, S172A, S187A (wt site at T146)
Lanes 7-8 : T18A, T146A, S172A, S187A (wt site at T154)
Lanes 9-10 : T18A, T146A, T154A, S187A (wt site at S172)
Lanes 11-12: T18A, T146A, T154A, S172A (wt site at S187)
Lanes 13-14: T18A, T146A, T154A, S172A, S187A
Alejandro Contreras, Baylor College of Medicine
Rabbit polyclonal to Histone H1 (phospho T18) (1/25).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab3595 (green). The DNA stained with DAPI is shown in red. (100x magnification).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"