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AETATPAPVEKSPAKKKATKKAAGAGAAK -C, corresponding to N terminal amino acids 7-35 of Human Histone H1.5
Our Abpromise guarantee covers the use of ab24175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50. PubMed: 19336418|
|WB||1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 22 kDa).|
|IP||Use at an assay dependent dilution. PubMed: 21383955|
All lanes : Histone H1.5 antibody (ab24175)
Lane 1 : Chromatin preparation from a breast cancer cell line
Lane 2 : recombinant human H1.0
Lane 3 : recombinant human H1.1
Lane 4 : recombinant human H1.2
Lane 5 : recombinant human H1.3
Lane 6 : recombinant human H1.4
Lane 7 : recombinant human H1.5
Panel B is a silver stain of the gel in panel A.
ICC/IF image of ab24175 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24175 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.