Overview

  • Product name
    Anti-Histone H1.5 antibody
    See all Histone H1.5 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.5
  • Specificity
    This antibody is specific for Histone H1.5. Reacts with histone H1 acid-extracted from cell lines such as breast cancer cell line T47D. Also recognizes H1 in a nuclear extract or chromatin preparation. H1.5 is present in many human cell lines.
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    AETATPAPVEKSPAKKKATKKAAGAGAAK -C

    , corresponding to N terminal amino acids 7-35 of Human Histone H1.5

  • Positive control
    • This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: HepG2.
  • General notes
    ab24175 does not appear to work in IF in human or mouse cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab24175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50. PubMed: 19336418
WB 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 22 kDa).
IP Use at an assay dependent dilution. PubMed: 21383955

Target

  • Function
    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 antibody
    • H1 histone family member 5 antibody
    • H1.5 antibody
    • H15 HUMAN antibody
    • H15_HUMAN antibody
    • H1B antibody
    • H1F5 antibody
    • H1s 3 antibody
    • Hist1h1b antibody
    • Histone 1 H1b antibody
    • Histone cluster 1 H1b antibody
    • Histone H1.5 antibody
    • Histone H1a antibody
    • Histone H1b antibody
    • Histone H1s 3 antibody
    • MGC126630 antibody
    • MGC126632 antibody
    see all

Anti-Histone H1.5 antibody images



  • Predicted band size : 22 kDa
    All lanes : Histone H1.5 antibody (ab24175)

    Lane 1 : Chromatin preparation from a breast cancer cell line
    Lane 2 : recombinant human H1.0
    Lane 3 : recombinant human H1.1
    Lane 4 : recombinant human H1.2
    Lane 5 : recombinant human H1.3
    Lane 6 : recombinant human H1.4
    Lane 7 : recombinant human H1.5

    Panel B is a silver stain of the gel in panel A.

    All lanes : Histone H1.5 antibody (ab24175)

    Lane 1 : Chromatin preparation from a breast cancer cell line
    Lane 2 : recombinant human H1.0
    Lane 3 : recombinant human H1.1
    Lane 4 : recombinant human H1.2
    Lane 5 : recombinant human H1.3
    Lane 6 : recombinant human H1.4
    Lane 7 : recombinant human H1.5

    Panel B is a silver stain of the gel in panel A.

  • ICC/IF image of ab24175 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24175 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Histone H1.5 antibody (ab24175)

This product has been referenced in:
  • Liao R & Mizzen CA Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation. Epigenetics Chromatin 10:29 (2017). Read more (PubMed: 28539972) »
  • Holmberg Olausson K  et al. NPM1 histone chaperone is upregulated in glioblastoma to promote cell survival and maintain nucleolar shape. Sci Rep 5:16495 (2015). Read more (PubMed: 26559910) »

See all 12 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (MEF)
Negative control
See Additional notes
Specification
MEF
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1%
Positive control
See Additional notes
Username

Mr. Massimo Petretich

Verified customer

Submitted Dec 30 2013

Thank you for contacting us. I have checked with the lab, but the protein is unfortunately not available for sale. Also, I checked Biocompare (www.biocompare.com), but could find only the Abcam peptides for Histone H1.5, not the full le...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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