Overview

  • Product name
    Anti-Histone H1.5 antibody
    See all Histone H1.5 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.5
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for Histone H1.5. Reacts with histone H1 acid-extracted from cell lines such as breast cancer cell line T47D. Also recognizes H1 in a nuclear extract or chromatin preparation. H1.5 is present in many human cell lines.
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    AETATPAPVEKSPAKKKATKKAAGAGAAK -C

    , corresponding to N terminal amino acids 7-35 of Human Histone H1.5

  • Positive control
    • This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab24175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50. PubMed: 19336418
WB 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 22 kDa).
IP Use at an assay dependent dilution. PubMed: 21383955

Target

  • Function
    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 antibody
    • H1 histone family member 5 antibody
    • H1.5 antibody
    • H15 HUMAN antibody
    • H15_HUMAN antibody
    • H1B antibody
    • H1F5 antibody
    • H1s 3 antibody
    • Hist1h1b antibody
    • Histone 1 H1b antibody
    • Histone cluster 1 H1b antibody
    • Histone H1.5 antibody
    • Histone H1a antibody
    • Histone H1b antibody
    • Histone H1s 3 antibody
    • MGC126630 antibody
    • MGC126632 antibody
    see all

Images

  • All lanes : Histone H1.5 antibody (ab24175)

    Lane 1 : Chromatin preparation from a breast cancer cell line
    Lane 2 : recombinant human H1.0
    Lane 3 : recombinant human H1.1
    Lane 4 : recombinant human H1.2
    Lane 5 : recombinant human H1.3
    Lane 6 : recombinant human H1.4
    Lane 7 : recombinant human H1.5

    Panel B is a silver stain of the gel in panel A.

    All lanes : Histone H1.5 antibody (ab24175)

    Lane 1 : Chromatin preparation from a breast cancer cell line
    Lane 2 : recombinant human H1.0
    Lane 3 : recombinant human H1.1
    Lane 4 : recombinant human H1.2
    Lane 5 : recombinant human H1.3
    Lane 6 : recombinant human H1.4
    Lane 7 : recombinant human H1.5

    Panel B is a silver stain of the gel in panel A.

  • ICC/IF image of ab24175 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24175 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Liao R & Mizzen CA Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation. Epigenetics Chromatin 10:29 (2017). Read more (PubMed: 28539972) »
  • Izquierdo-Bouldstridge A  et al. Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats. Nucleic Acids Res 45:11622-11642 (2017). Read more (PubMed: 28977426) »

See all 13 Publications for this product

Customer reviews and Q&As

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Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (MEF)
Negative control
See Additional notes
Specification
MEF
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1%
Positive control
See Additional notes
Username

Mr. Massimo Petretich

Verified customer

Submitted Dec 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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