Our Abpromise guarantee covers the use of ab18208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use 2 µg for 25 µg of chromatin.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 antibody
    • H1 histone family member 5 antibody
    • H1.5 antibody
    • H15 HUMAN antibody
    • H15_HUMAN antibody
    • H1B antibody
    • H1F5 antibody
    • H1s 3 antibody
    • Hist1h1b antibody
    • Histone 1 H1b antibody
    • Histone cluster 1 H1b antibody
    • Histone H1.5 antibody
    • Histone H1a antibody
    • Histone H1b antibody
    • Histone H1s 3 antibody
    • MGC126630 antibody
    • MGC126632 antibody
    see all

Anti-Histone H1.5 antibody - ChIP Grade images

  • Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab18208 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • All lanes : Anti-Histone H1.5 antibody - ChIP Grade (ab18208) at 0.5 µg/ml

    Lane 1 : Recombinant histone H1
    Lane 2 : Recombinant histone H1.1
    Lane 3 : Recombinant histone H1.2
    Lane 4 : Recombinant histone H1.3
    Lane 5 : Recombinant histone H1.4
    Lane 6 : Recombinant histone H1.5

    Predicted band size : 32 kDa
    Observed band size : 32 kDa

    This image is courtesy of Dr Albert Jordan and Monica Sancho, Center for Genomic Regulation (CRG). Recombinant H1 isoforms courtesy of Dr Nicole Happel.

  • ICC/IF image of ab18208 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18208, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab18208 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18208, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Histone H1.5 antibody - ChIP Grade (ab18208)

This product has been referenced in:
  • Laserna EJ  et al. Proteomic analysis of phosphorylated nuclear proteins underscores novel roles for rapid actions of retinoic acid in the regulation of mRNA splicing and translation. Mol Endocrinol 23:1799-814 (2009). Read more (PubMed: 19812389) »
  • Sancho M  et al. Depletion of human histone H1 variants uncovers specific roles in gene expression and cell growth. PLoS Genet 4:e1000227 (2008). WB ; Human . Read more (PubMed: 18927631) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing (12.5)
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted May 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MRC5VA Lung Fibroblasts)
Loading amount 15 µg
Specification MRC5VA Lung Fibroblasts
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 10 2011