Anti-Histone H1oo antibody (ab71580)

Overview

  • Product nameAnti-Histone H1oo antibody
    See all Histone H1oo primary antibodies
  • Description
    Rabbit polyclonal to Histone H1oo
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Histone H1oo (C terminal).
    Database link: Q8VIK3

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 32 kDa.Can be blocked with Histone H1oo peptide (ab194535).

Target

  • FunctionMay play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
  • Tissue specificityOocyte-specific.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localizationCytoplasm. Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member O oocyte specific antibody
    • H1FOO antibody
    • H1FOO_HUMAN antibody
    • Histone H1oo antibody
    • Oocyte-specific histone H1 antibody
    • Oocyte-specific linker histone H1 antibody
    • osH1 antibody
    see all

References for Anti-Histone H1oo antibody (ab71580)

ab71580 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hela, Hep G2)
Specification Hela, Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"