Anti-Histone H1oo antibody (ab87556)

Overview

  • Product nameAnti-Histone H1oo antibody
    See all Histone H1oo primary antibodies
  • Description
    Rabbit polyclonal to Histone H1oo
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H1oo aa 1-50 (N terminal). Immunogen is a synthetic peptide located within the following region: MAPGSVTSDISPSSTSTAGSSRSPESEKPGPSHGGVPPGGPSHSSLPVGR Within aa 1-50
    Database link: Q8IZA3

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
  • Tissue specificityOocyte-specific.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localizationCytoplasm. Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member O oocyte specific antibody
    • H1FOO antibody
    • H1FOO_HUMAN antibody
    • Histone H1oo antibody
    • Oocyte-specific histone H1 antibody
    • Oocyte-specific linker histone H1 antibody
    • osH1 antibody
    see all

Anti-Histone H1oo antibody images

  • Anti-Histone H1oo antibody (ab87556) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Gel concentration: 12%

References for Anti-Histone H1oo antibody (ab87556)

ab87556 has not yet been referenced specifically in any publications.

Product Wall

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H1.2 specific antibodies,
ab4086; http://www.abcam.com/Histone-H1-2-antibody-ChIP-Grade-ab4086.html. (we however haven't tested the specificity to other forms)
ab17677; http://www.abcam.com/Histone-H1-2-...

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Thank you for contacting us.

ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

ab83058; the sequence used is from P07305 http://...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - nuclear (Muscle)
Loading amount 100 µg
Specification Muscle
Gel Running Conditions Reduced Denaturing (12)
Blocking step Blocking Buffer (ready-to-use, Odyssey) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
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Submitted Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"