Overview

  • Product nameAnti-Histone H1t antibody
  • Description
    Rabbit polyclonal to Histone H1t
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Histone H1t aa 108-157 (internal sequence).
    Sequence:

    SFKLSKKVIPKSTRSKAKKSVSAKTKKLVLSRDSKSPKTAKTNKRAKKPR

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher-order structures.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Developmental stageThis histone is a testis-specific H1 variant that appears during meiosis in spermatogenesis.
  • Post-translational
    modifications
    Phosphorylated in early spermatids.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family, member T (testis specific) antibody
    • H1FT antibody
    • H1T antibody
    • H1T_HUMAN antibody
    • Hist1h1t antibody
    • Histone cluster 1, H1t antibody
    • Histone H1t antibody
    • Testicular H1 histone antibody
    see all

Anti-Histone H1t antibody images



  • Predicted band size : 22 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling HIST1H1T with ab81498 at 1.0µg/ml.
  • Anti-Histone H1t antibody (ab81498) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : 22 kDa

References for Anti-Histone H1t antibody (ab81498)

ab81498 has not yet been referenced specifically in any publications.

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