Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)

Overview

  • Product name
    Anti-Histone H2A (acetyl K5) antibody - ChIP Grade
    See all Histone H2A primary antibodies
  • Description
    Rabbit polyclonal to Histone H2A (acetyl K5) - ChIP Grade
  • Specificity
    Histone H2A acetylated at lysine 5.
  • Tested applications
    Suitable for: ChIP, ELISA, IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Caenorhabditis elegans, Drosophila melanogaster, Mammal, Asian toad
  • Immunogen

    Synthetic peptide corresponding to Histone H2A (acetyl K5) conjugated to Ovalbumin.
    Sequence:

    SGRGK(Ac)QGGKYC

  • Positive control
    • HL60 cells treated with butyrate. IHC-P: Human normal colon FFPE tissue sections.
  • General notes

    ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab1764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/800 - 1/2000. Detects a band of approximately 20 kDa.

Use 5% BSA in TBST to block.

IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H2A family.
  • Post-translational
    modifications
    The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • H2A antibody
    • H2A1B_HUMAN antibody
    • H2AFM antibody
    • HIST1H2A antibody
    • HIST1H2AE antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    see all

Anti-Histone H2A (acetyl K5) antibody - ChIP Grade images

  • IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC - Wholemount of Caenorhabditis elegans whole body labelling Histone H2A (acetyl K5) with ab1764. Sample was incubated with primary antibody (1/2500 in 3% BSA + 0.1% Triton X-100 in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Left - Histone H2A (acetyl K5) (green), Right - nuclei (DAPI, blue). 

    See Abreview

  • ab1764 staining Female Embryonic Stem cells differentiated for 7 days by immunocytochemistry. The antibody was used at a dilution of 1/200 and incubated with the cells for 1 hour. Bound antibody was detected with a FITC conjugated Goat anti-Rabbit polyclonal antibody.

    DAPI counterstain is shown pseudocoloured as white on the right of the image (b). Centric heterochromatin was underacetylated in differentiated cells (arrowhead in a). A pale staining chromosome was observed at high frequencies; previoulsy shown to be the inactive female X chromosome (arrow in a).

    This image is courtesy of an Abreview by Hugh Spotswood submitted on 13 February 2006.

    See Abreview



  • Observed band size : 15 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Ab1764 at a 1/1000 dilution in Western blot of human Osteosarcoma (U2OS) nuclear cell lysate.

    See Abreview

References for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)

This product has been referenced in:
  • Lau AC  et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin 9:44 (2016). ICC/IF . Read more (PubMed: 27777629) »
  • Min SW  et al. Critical role of acetylation in tau-mediated neurodegeneration and cognitive deficits. Nat Med 21:1154-62 (2015). Read more (PubMed: 26390242) »

See all 9 Publications for this product

Product Wall

Thank you for your enquiry and for forwarding your completed questionnaire to us. We are very sorry to hear that you are having problem with this antibody. We have looked through your protocol and would like to make some suggestions. 1. First o...

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As discussed over the telephone and in my response to your enquiry about ab2423, we will also refund you for this antibody. Needless to say, we will also be re-testing this product.

The antibody has not been tested for its reactivity with non actylated histones.

Thank you for your enquiry regarding our Rabbit Polyclonal to Histone H2A - Acetyl K5 ab1764 We can not guarantee that this antibody would specifically detect H2A on the inactive X chromosome. We are aware of MacroH2A1 and MacroH2A2 (see Hum Mol Gene...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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