Overview

  • Product nameAnti-Histone H2A-Bbd antibody
    See all Histone H2A-Bbd primary antibodies
  • Description
    Rabbit polyclonal to Histone H2A-Bbd
  • SpecificityKnown to detect the recombinant form of human Histone H2A BBD. However, no reaction has yet been demonstrated against the endogenous protein in histone preparations. We believe that this is because of the low abundance of this variant in such preparations.
  • Tested applicationsSuitable for: ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H2A-Bbd aa 50 to the C-terminus. Also SwissProt ID: P0C5Y9
    Database link: P0C5Z0
    (Peptide available as ab15024, ab15024, ab15024)

  • Positive control
    • Recombinant H2ABbd

Properties

Applications

Our Abpromise guarantee covers the use of ab4175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 3 µg/ml. Staining may not be specific for H2A.Bbd (see image).
WB 1/500. Detects a band of approximately 12.5 kDa (predicted molecular weight: 12.5 kDa). As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
ICC/IF Use at an assay dependent dilution. PubMed: 20943707

Target

  • FunctionAtypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis.
  • Tissue specificityPresent in mature sperm.
  • Sequence similaritiesBelongs to the histone H2A family.
  • DomainThe docking domain is responsible for the weaker heterodimerization with H2B.
  • Cellular localizationNucleus. Chromosome. Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • H2A Barr body deficient antibody
    • H2A Barr body-deficient antibody
    • H2A histone family member B antibody
    • H2A.Bbd antibody
    • H2AB2_HUMAN antibody
    • H2ABBD antibody
    • H2AFB antibody
    • H2AFB1 antibody
    • H2AFB2 antibody
    • H2AFB3 antibody
    • Histone H2A BBD antibody
    • Histone H2A-Bbd type 1 antibody
    • Histone H2A-Bbd type 2/3 antibody
    see all

Anti-Histone H2A-Bbd antibody images

  • H2A-Bbd (ab4175)

      
    CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*=  recombinant H2ABbd  (the numbers underneath the lanes are the number of nanograms loaded in each lane).


    The primary antibody dilution was 1:500 and the secondary was 1:3000.

    As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.

  • Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.

References for Anti-Histone H2A-Bbd antibody (ab4175)

This product has been referenced in:
  • Nashun B  et al. Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice. Development 137:3785-94 (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20943707) »
  • Lee JH  et al. Chromatin analysis of occluded genes. Hum Mol Genet 18:2567-74 (2009). ChIP ; Human . Read more (PubMed: 19380460) »

See all 2 Publications for this product

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