Recombinant
RabMAb

Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)

Overview

  • Product name
    Anti-Histone H2A (symmetric di methyl R11) antibody [E18766]
    See all Histone H2A primary antibodies
  • Description
    Rabbit monoclonal [E18766] to Histone H2A (symmetric di methyl R11)
  • Tested applications
    Suitable for: WB, PepArrmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H2A aa 1-100 (symmetric di methyl R11). The exact sequence is proprietary.
    Database link: P04908

  • Positive control
    • Human ovary; NT2/D1, HL60, NCCIT, F9, mESC, mouse ovary lysate, C2C12, 3T3-L1, HeLa and NIH 3T3 lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Production method: Hybridoma.
  • Clonality
    Monoclonal
  • Clone number
    E18766
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
PepArr Use at an assay dependent concentration.

Target

  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H2A family.
  • Post-translational
    modifications
    The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • FO108 antibody
    • H2A antibody
    • H2A1B_HUMAN antibody
    • H4 histone family 2 antibody
    • H4 histone family member N antibody
    • H4F2 antibody
    • H4FN antibody
    • HIST1H2AE antibody
    • HistH2A antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    see all

Images

  • All lanes : Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464) at 1/500 dilution

    Lane 1 : Human ovary lysate
    Lane 2 : NT2/D1(human malignant pluripotent embryonic carcinoma ) whole cell lysate
    Lane 3 : HL60 (Human promyelocytic leukemia cells) whole cell lysate
    Lane 4 : NCCIT(human pluripotent embryonic carcinoma) whole cell lysate
    Lane 5 : F9 (Mouse embryo testicular cancer cell line) whole cell lysate
    Lane 6 : Human placenta lysate
    Lane 7 : mESC (mouse embryo stem cell) whole cell lysate
    Lane 8 : Mouse ovary lysate
    Lane 9 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 10 : 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 11 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 12 : NIH 3T3 (Mouse embryo fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size : 14 kDa


    Exposure time : 3 minutes

    5% NFDM/TBST: Blocking and diluting buffer.

  • All batches of ab195464are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

References

ab195464 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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