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Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
Overview
Product name
Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade
See all Histone H2A.Z products (8) ...
Description
Sheep polyclonal to Histone H2A.Z (acetyl K4 + K7 + K11) - ChIP Grade
Tested applications
ChIP, IF, WBmore details
Cross reactivity
Reacts with
Mouse, Chicken, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Chicken H2A.Z.
AGGKAcAGKA cDSGKAcAKA KAC
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 14 kDa (predicted molecular weight: 13 kDa).
Target
Relevance
The conserved histone variant H2A.Z functions in euchromatin to antagonize the spread of heterochromatin. The mechanism by which histone H2A is replaced by H2A.Z in the nucleosome is unknown. However, the recently isolated SWR1 complex catalyzes an ATP-dependent histone exchange with specificity for the histone variant H2A.Z. This provides a new theme in chromatin remodeling mechanisms and an explanation of how histone variants are incorporated into the nucleosome outside of S phase. In connection with the isolation of histone variant specific chaperone complexes, we are beginning to see the mechanisms that mix the histone octamer with intriguing implications for epigenetic inheritance. During early mammalian development H2A.Z plays an essential, but unknown, function(s). It has been shown (Rangasamy et al., 2003) that undifferentiated mouse cells of the inner cell mass lack H2A.Z, but upon differentiation H2A.Z expression is switched on. Strikingly, H2A.Z is first targeted to pericentric hetero chromatin and then to other regions of the nucleus, but is excluded from the inactive X chromosome and the nucleolus.
Cellular localization
Nuclear
Alternative names
- H2AFZ antibody
- H2A histone family member Z antibody
- H2A.z antibody
see all
Database links
- Entrez Gene: 3015 Human
- Entrez Gene: 51788 Mouse
- Omim: 142763 Human
- SwissProt: P02272 Chicken
- SwissProt: P0C0S5 Human
- SwissProt: P0C0S6 Mouse
- SwissProt: P17317 Mouse
- SwissProt: Q3TFU6 Mouse
see all
Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade images:
Western blot - H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)
All lanes : Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)
Lane 1 : Butyrate-treated HeLa histones
Lane 2 : 15-day embryo chicken erythrocyte histones
Lane 3 : Recombinant H2A.Z
Lane 4 : Butyrate-treated HeLa histones
Lane 5 : 15-day embryo chicken erythrocyte histones
Lane 6 : Recombinant H2A.Z
Predicted band size : 13 kDa
Observed band size : 13 kDa
Prof Colyn Crane-Robinson
Western blot - H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)
All lanes : Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)
Lane 1 : Butyrate-treated HeLa histones
Lane 2 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.
Lane 3 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.
Lane 4 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.
Lane 5 : Butyrate-treated HeLa histones
Lane 6 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.
Lane 7 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.
Lane 8 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.
Predicted band size : 13 kDa
Observed band size : 13 kDa
Nucleosomes from 10-day chicken brain tissue were used in ChIP experiments. An acetic acid / urea / Triton gel was run and the corresponding western blot using ab18262 was used to compare proteins in the Bound chromatin fraction with those of the Input and Unbound fractions. In lane 8 there is a strong diffuse band corresponding to a hyper-acetylated H2A.Z.
Prof Colyn Crane-Robinson
References for Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)
This product has been referenced in:
- Sérandour AAet al. Epigenetic switch involved in activation of pioneer factor FOXA1-dependent enhancers. Genome Res 21:555-65 (2011). ChIP; Human.Read more (PubMed: 21233399) »
- Kelly TKet al. H2A.Z maintenance during mitosis reveals nucleosome shifting on mitotically silenced genes. Mol Cell 39:901-11 (2010). ChIP; Human.Read more (PubMed: 20864037) »
See all 5 publications for this product
Publishing research using ab18262? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"