Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
|
ab46666 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab18262? Please let us know so that we can cite the reference in this datasheet
ab18262 has been referenced in 6 publications.
Publishing research using ab18262? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)
Lane 1 : Butyrate-treated HeLa histones
Lane 2 : 15-day embryo chicken erythrocyte histones
Lane 3 : Recombinant H2A.Z
Lane 4 : Butyrate-treated HeLa histones
Lane 5 : 15-day embryo chicken erythrocyte histones
Lane 6 : Recombinant H2A.Z
Predicted band size : 13 kDa
Observed band size : 13 kDa
Prof Colyn Crane-Robinson
All lanes : Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)
Lane 1 : Butyrate-treated HeLa histones
Lane 2 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.
Lane 3 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.
Lane 4 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.
Lane 5 : Butyrate-treated HeLa histones
Lane 6 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.
Lane 7 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.
Lane 8 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.
Predicted band size : 13 kDa
Observed band size : 13 kDa
Nucleosomes from 10-day chicken brain tissue were used in ChIP experiments. An acetic acid / urea / Triton gel was run and the corresponding western blot using ab18262 was used to compare proteins in the Bound chromatin fraction with those of the Input and Unbound fractions. In lane 8 there is a strong diffuse band corresponding to a hyper-acetylated H2A.Z.
Prof Colyn Crane-Robinson
1
Call 01223 696 000 or contact us