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Synthetic peptide corresponding to Human Histone H2A.Z.
This antibody clone is manufactured by Abcam.
Our Abpromise guarantee covers the use of ab80150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).|
|Flow Cyt||Use 1µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
ab80150 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
ab80150 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"