Our Abpromise guarantee covers the use of ab18263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|ChIP||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).|
This image is courtesy of Prof Colyn Crane-Robinson
This image is courtesy of Prof Colyn Crane-RobinsonNucleosomes from 10-day chicken brain tissue were used in ChIP experiments. An acetic acid / urea / Triton gel was run and the corresponding western blot using ab18263 was used to compare proteins in the Bound chromatin fraction with those of the Input and Unbound fractions. In lane 10 there is a strong band corresponding to a hyper-acetylated H2A.Z.