Anti-Histone H2A.Z antibody - ChIP Grade (ab4626)

Overview

  • Product nameAnti-Histone H2A.Z antibody - ChIP Grade
    See all Histone H2A.Z primary antibodies
  • Description
    Rabbit polyclonal to Histone H2A.Z - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WB, IPmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae Histone H2A.Z aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q12692
    (Peptide available as ab17826)

  • Positive control
    • This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab4626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
IP Use at an assay dependent dilution. PubMed: 17373856

Target

  • FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • Sequence similaritiesBelongs to the histone H2A family.
  • Post-translational
    modifications
    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Alternative names
    • H2A histone family member Z antibody
    • H2A.z antibody
    • H2A/z antibody
    • H2afz antibody
    • H2AZ antibody
    • H2AZ_HUMAN antibody
    • Histone H2A.Z antibody
    • MGC117173 antibody
    see all

Anti-Histone H2A.Z antibody - ChIP Grade images

  • Anti-Histone H2A.Z antibody - ChIP Grade (ab4626) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa


    Exposure time : 20 minutes
    Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ab4626 used in ChIP.
    Nuclear lysate prepared from S cerevisiae S288c was used.
    Cross linking (X-ChIP) was performed for 15 seconds with 1% formaldehyde.
    The detection step was performed using Real-time PCR.
    ab4626 was used at a 1/100 dilution for 4 hours at 4°C.

    See Abreview

References for Anti-Histone H2A.Z antibody - ChIP Grade (ab4626)

This product has been referenced in:
  • Zhang H  et al. Uncoupling transcription from covalent histone modification. PLoS Genet 10:e1004202 (2014). ChIP . Read more (PubMed: 24722509) »
  • Yu Y  et al. Histone variant Htz1 promotes histone H3 acetylation to enhance nucleotide excision repair in Htz1 nucleosomes. Nucleic Acids Res 41:9006-19 (2013). Read more (PubMed: 23925126) »

See all 12 Publications for this product

Product Wall


I have aligned both protein sequences - the overall homology is 77%, but for the immunogen region (N-term.) there is unfortunately no homology. Thus, this antibody would not work with S.pombe.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Detection step Real-time PCR
Sample Saccharomyces cerevisiae Cell lysate - nuclear (S288c)
Specification S288c
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Fromaldehyde
Username

Mr. Harsh Ja

Verified customer

Submitted Aug 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (15% gel)
Sample Saccharomyces cerevisiae Cell lysate - nuclear (S288c)
Specification S288c
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Mr. Harsh Ja

Verified customer

Submitted Aug 09 2011

Application ChIP on chip
Detection step Microarray
Sample Saccharomyces cerevisiae Cell lysate - nuclear (BY4741 background)
Specification BY4741 background
Negative control No antibody control
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 20 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Detection step Semiquantitative PCR
Sample Saccharomyces cerevisiae Cell lysate - nuclear (BY4741 background)
Specification BY4741 background
Negative control No antibody control
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control Htz1 deletion
Username

Abcam user community

Verified customer

Submitted May 31 2011

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (15% gel)
Sample Saccharomyces cerevisiae Cell lysate - whole cell (Yeast BY4741 background)
Specification Yeast BY4741 background
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted May 26 2011

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Saccharomyces cerevisiae Cell lysate - whole cell (S288c background)
Specification S288c background
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"