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Synthetic peptide corresponding to Histone H2A.Z.
Our Abpromise guarantee covers the use of ab4462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 13.5 kDa).|
ab4462 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
ab4462 has not yet been referenced specifically in any publications.