Purification notesab61228 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
1/50 - 1/100.
Use at an assay dependent concentration.
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H2B family.
Post-translational modificationsMonoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H2B (acetyl K12) antibody (ab61228) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.
Western blot - Histone H2B (acetyl K12) antibody (ab61228)
All lanes : Anti-Histone H2B (acetyl K12) antibody (ab61228) at 1/500 dilution
Lane 1 : COS7 cells treated
with TSA (400nM, 24hours), without acetylated peptide Lane 2 : COS7 cells treated
with TSA (400nM, 24hours) with acetylated peptide
Predicted band size : 14 kDa Observed band size : 14 kDa
References for Anti-Histone H2B (acetyl K12) antibody (ab61228)
This product has been referenced in:
Hait NC et al. Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory. Nat Neurosci17:971-80 (2014).
Read more (PubMed: 24859201) »