Overview

  • Product nameAnti-Histone H2B (acetyl K12) antibody
    See all Histone H2B primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (acetyl K12)
  • Specificityab61228 detects endogenous levels of Histone H2B only when acetylated at lysine 12. (Human: Lys12; Mouse: Lys12; Rat: Lys12)
  • Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Histone H2B (acetyl K12). The exact sequence is proprietary.
    Sequence:

    PKK(Ac)GS

  • Positive control
    • Human lung carcinoma tissue (IHC-P). HeLa cells (ICC/IF). COS7 cells treated with TSA (400nM, 24hours) (WB).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab61228 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H2B family.
  • Post-translational
    modifications
    Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
    Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H2B 1A antibody
    • H2B antibody
    • H2B histone family antibody
    • H2B2f antibody
    • H2B2F_HUMAN antibody
    • H2Ba antibody
    • H2Bf antibody
    • HIST2H2BF antibody
    • histone H2B antibody
    • histone H2B type 1 antibody
    • Histone H2B type 2-F antibody
    • MGC131639 antibody
    see all

Anti-Histone H2B (acetyl K12) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H2B (acetyl K12) antibody (ab61228) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.
  • All lanes : Anti-Histone H2B (acetyl K12) antibody (ab61228) at 1/500 dilution

    Lane 1 : COS7 cells treated with TSA (400nM, 24hours), without acetylated peptide
    Lane 2 : COS7 cells treated with TSA (400nM, 24hours) with acetylated peptide


    Predicted band size : 14 kDa
    Observed band size : 14 kDa

References for Anti-Histone H2B (acetyl K12) antibody (ab61228)

This product has been referenced in:
  • Hait NC  et al. Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory. Nat Neurosci 17:971-80 (2014). Mouse . Read more (PubMed: 24859201) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"