Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335)

Overview

  • Product nameAnti-Histone H2B (acetyl K15) antibody [EP955Y]
    See all Histone H2B primary antibodies
  • Description
    Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15)
  • SpecificityThis antibody only detects Histone H2B acetylated on Lysine 15.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B aa 1-100 (acetyl K15).

  • Positive control
    • HeLa cell lysate or human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP955Y
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab62335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.
WB 1/2000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • Sequence similaritiesBelongs to the histone H2B family.
    • Post-translational
      modifications
      Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
      Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
    • Cellular localizationNucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • H2B 1A antibody
      • H2B antibody
      • H2B histone family antibody
      • H2B2f antibody
      • H2B2F_HUMAN antibody
      • H2Ba antibody
      • H2Bf antibody
      • HIST2H2BF antibody
      • histone H2B antibody
      • histone H2B type 1 antibody
      • Histone H2B type 2-F antibody
      • MGC131639 antibody
      see all

    Anti-Histone H2B (acetyl K15) antibody [EP955Y] images

    • All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335) at 1/2000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with TSA)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
    • ab62335 (1/500) staining Histone H2B (acetyl K15) in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

      See Abreview

    References for Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - whole cell (HEK293T cells)
    Specification HEK293T cells
    Total protein in input 5 µg
    Username

    Abcam user community

    Verified customer

    Submitted Dec 20 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (Mouse thymus tissue)
    Specification Mouse thymus tissue
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 20 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (15%)
    Sample Rat Cell lysate - other (Chromatin extracted from thymocytes)
    Specification Chromatin extracted from thymocytes
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 20 2013

    Application Immunocytochemistry
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample Mouse Cultured Cells (Mouse embryonic neural stem cells)
    Specification Mouse embryonic neural stem cells
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 20 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12.5%)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic neural stem cells)
    Specification Mouse embryonic neural stem cells
    Treatment Withdraw 10ug/ml FGF2
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 20 2013



    1.) I can confirm that this was not tested experimentally. The exact immunogenic sequence of ab139383 is 15 amino acids long. Alignment of the immunogenic sequence with the sequence for P70696 (mouse TH2B) is as follows:

    80.0% iden...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Nov 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"