• Product name
    Anti-Histone H2B (acetyl K16) antibody
    See all Histone H2B primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (acetyl K16)
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ChIP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H2B, acetylated at K16.

    (Peptide available as ab41879.)

  • Positive control
    • ab40977 gave a positive signal in the following lysate: HeLa Histone Preparation Nuclear Lysate - Butyrated treated


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab40977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
ChIP Use a concentration of 4 µg/ml. PubMed: 20693536
ICC/IF Use a concentration of 1 µg/ml.



  • All lanes : Anti-Histone H2B (acetyl K16) antibody (ab40977) at 1 µg/ml

    Lane 1 : HeLa Histone Preparation Nuclear Lysate
    Lane 2 : HeLa Histone Preparation Nuclear Lysate - Butyrated treated

    Lane 3 : HeLa Histone Preparation Nuclear Lysate with Human Histone H2B (acetyl K16) peptide (ab41879) at 1 µg/ml
    Lane 4 : HeLa Histone Preparation Nuclear Lysate - Butyrated treated
    with Human Histone H2B (acetyl K16) peptide (ab41879) at 1 µg/ml
    Lane 5 : HeLa Histone Preparation Nuclear Lysate with Human Histone H2B peptide (ab35793) at 1 µg/ml
    Lane 6 : HeLa Histone Preparation Nuclear Lysate - Butyrated treated
    with Human Histone H2B peptide (ab35793) at 1 µg/ml

    Lysates/proteins at 2.5 µg per lane.

    All lanes : HRP Conjugated Goat Anti-Rabbit IgG at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 16 kDa (why is the actual band size different from the predicted?)

  • ICC/IF image of ab40977 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40977, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of ab40977 staining in cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40977, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Navarro-Costa P  et al. Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling. Nat Commun 7:12331 (2016). IF ; Drosophila melanogaster . Read more (PubMed: 27507044) »
  • Singh P  et al. Coordinated allele-specific histone acetylation at the differentially methylated regions of imprinted genes. Nucleic Acids Res 38:7974-90 (2010). ChIP ; Mouse . Read more (PubMed: 20693536) »

See all 2 Publications for this product

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