Anti-Histone H2B (acetyl K5) antibody (ab61227)

Overview

  • Product nameAnti-Histone H2B (acetyl K5) antibody
    See all Histone H2B primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (acetyl K5)
  • Specificityab61227 detects Histone H2B only when acetylated at lysine 5.
  • Tested applicationsSuitable for: ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Histone H2B (acetyl K5). The exact sequence is proprietary.
    Sequence:

    PAK(Ac)SA


    Database link: P57053

  • Positive control
    • Human breast carcinoma tissue. HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab61227 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H2B family.
  • Post-translational
    modifications
    Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
    Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H2B 1A antibody
    • H2B antibody
    • H2B histone family antibody
    • H2B2f antibody
    • H2B2F_HUMAN antibody
    • H2Ba antibody
    • H2Bf antibody
    • HIST2H2BF antibody
    • histone H2B antibody
    • histone H2B type 1 antibody
    • Histone H2B type 2-F antibody
    • MGC131639 antibody
    see all

Anti-Histone H2B (acetyl K5) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H2B (acetyl K5) antibody (ab61227) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising acetylated peptide.
  • Immunofluorescence analysis of HeLa cells, using Histone H2B (acetyl K5) antibody (ab61227) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising acetylated peptide.

References for Anti-Histone H2B (acetyl K5) antibody (ab61227)

ab61227 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"