Purification notesab61227 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/500 - 1/1000.
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H2B family.
Post-translational modificationsMonoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H2B (acetyl K5) antibody (ab61227) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising acetylated peptide.
Immunofluorescence analysis of HeLa cells, using Histone H2B (acetyl K5) antibody (ab61227) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising acetylated peptide.
References for Anti-Histone H2B (acetyl K5) antibody (ab61227)
This product has been referenced in:
Lee JR et al. High-Resolution Analysis of Antibodies to Post-Translational Modifications Using Peptide Nanosensor Microarrays. ACS Nano10:10652-10660 (2016).
Read more (PubMed: 27636738) »