This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Histone H2B (di methyl K43) antibody (ab4629)

Overview

  • Product nameAnti-Histone H2B (di methyl K43) antibody
    See all Histone H2B primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (di methyl K43)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Species reactivity

    Predicted to work with: Mouse, Rat, Chicken, Human, Xenopus laevis, Drosophila melanogaster, Duck
  • Immunogen

    Synthetic peptide corresponding to Human Histone H2B aa 1-100 (di methyl K43) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q16778
    (Peptide available as ab23555)

  • Positive control
    • Uncertain of correct test conditions for this histone modification.

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

This antibody gave a positive result in ELISA against the immunising peptide (ab23555). It gave a negative result in ELISA against the non-modified equivalent peptide (ab13214). This indicates that it is specific for the modified peptide. See figure below.

Target

  • RelevanceCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • H2B K antibody
    • H2B type 12 antibody
    • H2B/b antibody
    • H2B/c antibody
    • H2B/d antibody
    • H2B/e antibody
    • H2B/f antibody
    • H2B/j antibody
    • H2B/n antibody
    • H2B/q antibody
    • H2B/r antibody
    • H2B/s antibody
    • HIRA-interacting protein 1 antibody
    • HIRA-interacting protein 2 antibody
    • Histone H2B type 1-B antibody
    • Histone H2B type 1-C/E/F/G/I antibody
    • Histone H2B type 1-D antibody
    • Histone H2B type 1-H antibody
    • Histone H2B type 1-J antibody
    • Histone H2B type 1-K antibody
    • Histone H2B type 1-L antibody
    • Histone H2B type 1-M antibody
    • Histone H2B type 1-N antibody
    • Histone H2B type 1-O antibody
    • Histone H2B type 2-E antibody
    • Histone H2B type 2-F antibody
    • Histone H2B type 3-B antibody
    • Histone H2B type F-S antibody
    • Histone H2B-GL105 antibody
    • Histone H2B.1 A antibody
    • Histone H2B.1 antibody
    • Histone H2B.1 B antibody
    • Histone H2B.2 antibody
    • Histone H2B.a antibody
    • Histone H2B.b antibody
    • Histone H2B.c antibody
    • Histone H2B.d antibody
    • Histone H2B.e antibody
    • Histone H2B.f antibody
    • Histone H2B.g antibody
    • Histone H2B.h antibody
    • Histone H2B.j antibody
    • Histone H2B.k antibody
    • Histone H2B.l antibody
    • Histone H2B.n antibody
    • Histone H2B.r antibody
    • Histone H2B.s antibody
    see all

Anti-Histone H2B (di methyl K43) antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • This antibody gave a positive result in ELISA against the immunising peptide (ab23555) and a negative result using the non-modified equivalent peptide (ab13214). This indicates that it is specific for the modified peptide.

    Blue line: ELISA against immunising peptide ab23555
    Grey line: ELISA against non-modified peptide ab13214

References for Anti-Histone H2B (di methyl K43) antibody (ab4629)

ab4629 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"