This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Histone H2B (hydroxy P10) antibody (ab30865)


  • Product nameAnti-Histone H2B (hydroxy P10) antibody
    See all Histone H2B primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (hydroxy P10)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Species reactivity
    Not yet tested in other species. Predicted to react with a wide range of species due to sequence homology.
  • Immunogen

    Synthetic peptide corresponding to Human Histone H2B aa 1-100 (hydroxy P10) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q16778
    (Peptide available as ab32105)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

This antibody gave a positive result in ELISA against the immunizing peptide (ab32105). It gave a negative result in ELISA against the non-modified equivalent peptide (ab13336).


  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
  • Sequence similaritiesBelongs to the histone H2B family.
  • Post-translational
    Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
    Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
    GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Alternative names
    • GL105 antibody
    • H2B antibody
    • H2B histone family member Q antibody
    • H2B-GL105 antibody
    • H2B.1 antibody
    • H2B/q antibody
    • H2B2E_HUMAN antibody
    • H2BFQ antibody
    • H2BGL105 antibody
    • H2BQ antibody
    • HIST2H2BE antibody
    • Histone 2 H2be antibody
    • histone cluster 2, H2be antibody
    • histone H2B GL105 antibody
    • histone H2B type 2 E antibody
    • Histone H2B type 2-E antibody
    • histone H2B type 2E antibody
    • Histone H2B-GL105 antibody
    • Histone H2B.q antibody
    • histone H2BGL105 antibody
    see all

Anti-Histone H2B (hydroxy P10) antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • This antibody gave a positive result in ELISA against the immunizing peptide (ab32105). It gave a negative result in ELISA against the non-modified equivalent peptide (ab13336). This indicates that it is specific for the modified peptide.   

References for Anti-Histone H2B (hydroxy P10) antibody (ab30865)

ab30865 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Nov 18 2011