Recombinant
RabMAb

Anti-Histone H2B (phospho T129) antibody [EPR18095] - ChIP Grade (ab188292)

Overview

  • Product name
    Anti-Histone H2B (phospho T129) antibody [EPR18095] - ChIP Grade
    See all Histone H2B primary antibodies
  • Description
    Rabbit monoclonal [EPR18095] to Histone H2B (phospho T129) - ChIP Grade
  • Host species
    Rabbit
  • Tested applications
    Suitable for: PepArr, WB, ChIPmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Saccharomyces cerevisiae Histone H2B (yeast) aa 100 to the C-terminus (phospho T129). The exact sequence is proprietary.
    Database link: P02293

  • Positive control
    • WB: Saccharomyces cerevisiae treated with 2 mg/ml Methyl methanesulfonate for 1 hour whole cell lysate. ChIP: Chromatin prepared from Saccharomyces cerevisiae cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
PepArr Use at an assay dependent concentration.
WB 1/100. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ChIP Use 2 µg for 25 µg of chromatin.

Target

  • Relevance
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved.
  • Cellular localization
    Nuclear
  • Alternative names
    • H2b.1A antibody
    • HIST1H2BD antibody
    • HIST1H2BH antibody
    • HIST1H2BL antibody
    • HIST1H2BM antibody
    • HIST1H2BN antibody
    • HIST2H2BE antibody
    • Histone H2B antibody
    • Histone H2B type 1-D antibody
    • Histone H2B type 1-H antibody
    • Histone H2B type 1-L antibody
    • Histone H2B type 1-M antibody
    • Histone H2B type 1-N antibody
    • Histone H2B type 2-E antibody
    see all

Images

  • All batches of ab188292 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
     
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
     
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • All lanes : Anti-Histone H2B (phospho T129) antibody [EPR18095] - ChIP Grade (ab188292) at 1/100 dilution

    Lane 1 : Untreated Saccharomyces cerevisiae whole cell lysate
    Lane 2 : Saccharomyces cerevisiae treated with 2 mg/ml Methyl methanesulfonate for 1 hour whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were treated with MMS at 2mg/ml for 1 h. Treated and non-treated Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab188292 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

References

ab188292 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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