Anti-Histone H2B (yeast) antibody (ab188271)

Overview

  • Product name
    Anti-Histone H2B (yeast) antibody
    See all Histone H2B (yeast) primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (yeast)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Schizosaccharomyces pombe
    Predicted to work with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Schizosaccharomyces pombe Histone H2B (yeast) aa 2-17 (N terminal).
    Sequence:

    SAAEKKPASKAPAGKA


    Database link: P04913

  • Positive control
    • WB: S. Pombe cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab188271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 14 kDa.
IP Use at an assay dependent concentration.

(CHIP assay)

Target

  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H2B family.
  • Post-translational
    modifications
    Monoubiquitinated by the RAD6/UBC2-BRE1 complex to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and H3K79me formation. H2BK123ub1 also modulates the formation of double-strand breaks during meiosis and is a prerequisite for DNA-damage checkpoint activation. Deubiquitination is performed by UBP8 in presence of SGF11.
    Phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase. May be correlated with chromosome condensation. H2BS10ph is also formed after H(2)O(2) treatment, and is a step leading to apoptosis.
    Acetylated by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. H2BK16ac can also be formed by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
    Sumoylation to form H2BK6su or H2BK7su, and probably also H2BK16su or H2BK17su, occurs preferentially near the telomeres and represses gene transcription.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
    • Alternative names
      • H2B1_YEAST antibody
      • Histone H2B.1 antibody
      • Histone H2B.2 antibody
      • HTB 1 antibody
      • HTB 2 antibody
      • HTB1 antibody
      • Htb1p antibody
      • HTB2 antibody
      • Htb2p antibody
      • SPT 12 antibody
      • SPT12 antibody
      • Suppressor of Ty protein 12 antibody
      see all

    Images

    • Anti-Histone H2B (yeast) antibody (ab188271) at 1/1000 dilution + S. Pombe cell extracts.

      Predicted band size: 14 kDa



      The 17 kDa and 24~25 kDa bands correspond to the unmodified and the mono-ubiquitinated histone H2B, respectively.

    References

    ab188271 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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