Anti-Histone H2B (yeast) (phospho T129) antibody (ab92973)

Overview

  • Product name
    Anti-Histone H2B (yeast) (phospho T129) antibody
    See all Histone H2B (yeast) primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (yeast) (phospho T129)
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae Histone H2B (yeast) aa 100 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab140408)

  • Positive control
    • This antibody gave a positive signal in both Exponentially Growing Wild Type Yeast and Phleomycin Treated Yeast lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).

Target

  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H2B family.
  • Post-translational
    modifications
    Monoubiquitinated by the RAD6/UBC2-BRE1 complex to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and H3K79me formation. H2BK123ub1 also modulates the formation of double-strand breaks during meiosis and is a prerequisite for DNA-damage checkpoint activation. Deubiquitination is performed by UBP8 in presence of SGF11.
    Phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase. May be correlated with chromosome condensation. H2BS10ph is also formed after H(2)O(2) treatment, and is a step leading to apoptosis.
    Acetylated by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. H2BK16ac can also be formed by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
    Sumoylation to form H2BK6su or H2BK7su, and probably also H2BK16su or H2BK17su, occurs preferentially near the telomeres and represses gene transcription.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
    • Alternative names
      • H2B1_YEAST antibody
      • Histone H2B.1 antibody
      • Histone H2B.2 antibody
      • HTB 1 antibody
      • HTB 2 antibody
      • HTB1 antibody
      • Htb1p antibody
      • HTB2 antibody
      • Htb2p antibody
      • SPT 12 antibody
      • SPT12 antibody
      • Suppressor of Ty protein 12 antibody
      see all

    Anti-Histone H2B (yeast) (phospho T129) antibody images

    • All lanes : Anti-Histone H2B (yeast) (phospho T129) antibody (ab92973) at 1 µg/ml

      Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
      Lane 2 : Phleomycin Treated WT Yeast Whole Cell Lysate
      Lane 3 : Exponentially Growing WT Yeast Whole Cell Lysate with Immunising peptide at 1 µg/ml
      Lane 4 : Phleomycin Treated WT Yeast Whole Cell Lysate with Immunising peptide at 1 µg/ml
      Lane 5 : Exponentially Growing WT Yeast Whole Cell Lysate with Control peptide at 1 µg/ml
      Lane 6 : Phleomycin Treated WT Yeast Whole Cell Lysate with Control peptide at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 14 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 16 kDa,160 kDa,180 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

    References for Anti-Histone H2B (yeast) (phospho T129) antibody (ab92973)

    This product has been referenced in:
    • Lee CS  et al. Dynamics of yeast histone H2A and H2B phosphorylation in response to a double-strand break. Nat Struct Mol Biol 21:103-9 (2014). WB, ChIP . Read more (PubMed: 24336221) »

    See 1 Publication for this product

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