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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H3, acetylated at K122.
(Peptide available as ab34466.)
Our Abpromise guarantee covers the use of ab33309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 23415232|
|ChIP||Use at an assay dependent concentration. PubMed: 23415232|
|CHIPseq||Use at an assay dependent concentration. PubMed: 23415232|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (acetyl K122) peptide (ab34466).|
|ICC/IF||Use a concentration of 1 µg/ml.|
ICC/IF image of ab33309 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33309, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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