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Does not react withMouse
A synthetic acetyl-specific peptide corresponding to residues around Lysine 14 of Histone H3
Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.
Our Abpromise guarantee covers the use of ab52946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 19188451|
|WB||1/2000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/250 - 1/500.|
IHC - Wholemount of Caenorhabditis elegans embryo labelling Histone H3 with ab52946. Sample was incubated with primary antibody (1/200 in TBS + 5% BSA + 0.1% Triton-X 100) for 12 hours at 4°C. ab150077, an Alexa Flour® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
ab52946 immunoprecipitating Histone H3 (acetyl K14) in Drosophila melanogaster embryo whole tissue lysate. 500µg of tissue lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 2 hours at 4°C. For western blotting ab52946 (1/20000) was used to confirm successful immunoprecipation.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"