Histone H3 (acetyl K18) Quantification Kit (Fluorometric) (ab115109)

Overview

  • Product nameHistone H3 (acetyl K18) Quantification Kit (Fluorometric)
    See all Histone H3 (K18 acetylation) kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    0.4 ng/well
  • Range
    5 ng/well - 2000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Lysine 18 at histone H3, along with K9 and K14, are primary acetylated sites of histone H3. Histone H3 (acetyl K18) is tightly involved in cell cycle regulation, cell proliferation and apoptosis. It also correlates with transcription activation.

    Abcam's Histone H3 (acetyl K18) Quantification Kit (Fluorometric) (ab115109) is a convenient package of tools that allows the experimenter to measure global acetylation of histone H3K18 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H3K18 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Notes

     

     

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115109 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (acetyl K18) Quantification Kit (Fluorometric) (ab115109)

    ab115109 has not yet been referenced specifically in any publications.

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