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Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone H3 (acetyl K36) is a conserved modification, which functions in transcription. It is also tightly involved in cell cycle regulation, cell proliferation and apoptosis.
Abcam's Histone H3 (acetyl K36) Quantification Kit (Fluorometric) (ab115113) allows the experimenter to measure global acetylation of histone H3K36 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H3K36 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Fluoro Developer||1 x 12µl||1 x 24µl|
|Fluoro Dilution||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 12µl||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115113 in the following tested applications.
|Functional Studies||Use at an assay dependent concentration.|
ab115113 has not yet been referenced specifically in any publications.