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Synthetic peptide corresponding to Human Histone H3 (acetyl K9 + K14 + K18 + K23 + K27).
Our Abpromise guarantee covers the use of ab47915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 20080953
Use 10uL per ChIP experiment
|WB||1/500 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).|
ICC/IF image of ab47915 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47915,1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Histone H3ac (pan-acetyl) antibody tested by ChIP. ChIP was performed using a High Sensitivity Kit with 5 µg of chromatin from HeLa cells and 10 µl of Histone H3ac antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using a normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
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