Anti-Histone H3 (acetyl K9, phospho S10) antibody [APH3-64] (ab12180)

Overview

  • Product nameAnti-Histone H3 (acetyl K9, phospho S10) antibody [APH3-64]
    See all Histone H3 primary antibodies
  • Description
    Mouse monoclonal [APH3-64] to Histone H3 (acetyl K9, phospho S10)
  • SpecificityStaining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide.
  • Tested applicationsSuitable for: WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, a wide range of other species
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography. The resulting IgG fraction is further purified by absorption on the unmodified histone H3 peptide (human, amino acids 7-20).
  • ClonalityMonoclonal
  • Clone numberAPH3-64
  • MyelomaNS1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17 kDa.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • RelevanceHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination, are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by the modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Phosphorylation, particularly that of histones H1 and H3, has long been implicated in chromosome condensation during mitosis. However, converging evidence suggests that H3 phosphorylation (specifically serine 10) is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. The potential importance of the serine 10 phosphorylation mark in H3 is strengthened by the finding that MSK1, a kinase activated by growth factor and stress stimuli, also phosphorylates H3 in vitro. H3 phosphorylation at serine 10 in conjunction with phosphorylation at serine 28, is also required for proper segregation and condensation of chromosomes during mitosis and meiosis.
  • Database links
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  • Alternative names
    • H3 3 like sequence MH921 antibody
    • H3 3A antibody
    • H3 histone antibody
    • H3 histone family member E pseudogene antibody
    • H3F3 antibody
    • H3F3A antibody
    • HIST3H3 antibody
    • Histone H3 3 pseudogene antibody
    see all

References for Anti-Histone H3 (acetyl K9, phospho S10) antibody [APH3-64] (ab12180)

This product has been referenced in:
  • Landsman L  et al. Elevated Hedgehog/Gli signaling causes beta-cell dedifferentiation in mice. Proc Natl Acad Sci U S A 108:17010-5 (2011). IHC-P ; Mouse . Read more (PubMed: 21969560) »

See 1 Publication for this product

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The immunogen for Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in mouse was synthetic, acetylated and phosphorylated histone H3 peptide (amino acids 7-20, Ac-Lys9, pSer10) corres...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"