Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade (ab12181)

Overview

  • Product name
    Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade
    See all Histone H3 primary antibodies
  • Description
    Rabbit polyclonal to Histone H3 (acetyl K9, phospho S10) - ChIP Grade
  • Specificity
    Staining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide.
  • Tested applications
    Suitable for: ICC/IF, ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human, Drosophila melanogaster
    Predicted to work with: Rat, Cow, Xenopus laevis, Caenorhabditis elegans, a wide range of other species
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • interphase HepG2 cells (see Abreview)
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography. The resulting IgG fraction is further purified by absorption on the unmodified histone H3 peptide (human, amino acids 7-20).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
ChIP Use at an assay dependent concentration. PubMed: 17675409
WB 1/1000. Detects a band of approximately 17 kDa.

Target

  • Relevance
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination, are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by the modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Phosphorylation, particularly that of histones H1 and H3, has long been implicated in chromosome condensation during mitosis. However, converging evidence suggests that H3 phosphorylation (specifically serine 10) is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. The potential importance of the serine 10 phosphorylation mark in H3 is strengthened by the finding that MSK1, a kinase activated by growth factor and stress stimuli, also phosphorylates H3 in vitro. H3 phosphorylation at serine 10 in conjunction with phosphorylation at serine 28, is also required for proper segregation and condensation of chromosomes during mitosis and meiosis.
  • Database links
    see all
  • Alternative names
    • H3 3 like sequence MH921 antibody
    • H3 3A antibody
    • H3 histone antibody
    • H3 histone family member E pseudogene antibody
    • H3F3 antibody
    • H3F3A antibody
    • HIST3H3 antibody
    • Histone H3 3 pseudogene antibody
    see all

Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade images

  • This image was kindly supplied as part of the review submitted by Monika Harrer.
  • All lanes : Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade (ab12181)

    Lane 1 : Whole cell extract of NIH3T3 cells
    Lane 2 : Whole cell extract of NIH3T3 cells with [Ac-Lys9, pSer10] Histone H3 peptide
    Lane 3 : Whole cell extract of NIH3T3 cells with [non-Ac, non-phos] Histone H3 peptide
    Lane 4 : Whole cell extract of NIH3T3 cells with [Ac-Lys9] Histone H3 peptide
    Lane 5 : Whole cell extract of NIH3T3 cells with [pSer10] Histone H3 peptide

    Secondary
    Alkaline phosphatase conjugated Goat anti-Rabbit developed with a colorimetric substrate

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    Inhibition is shown in Lane 2.

References for Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade (ab12181)

This product has been referenced in:
  • Wiersma M  et al. Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes. Epigenetics Chromatin 9:52 (2016). ChIP ; Human . Read more (PubMed: 27895715) »
  • Rothbart SB  et al. An Interactive Database for the Assessment of Histone Antibody Specificity. Mol Cell 59:502-11 (2015). Read more (PubMed: 26212453) »

See all 13 Publications for this product

Product Wall

Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Embryo (Fruit fly embryo)
Specification
Fruit fly embryo
Username

Abcam user community

Verified customer

Submitted Nov 13 2013

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (Human Hela cells)
Specification
Human Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (HeLa)
Specification
HeLa
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
GAPDH promoter
Negative control
bglobin promoter
Username

Abcam user community

Verified customer

Submitted Jul 22 2010

I have double checked with my colleagues and we believe that the antibody will not recognise singly modified H3, as there was no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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