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ChIP - Histone H3 antibody - ChIP Grade (ab12079)
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- Histone H3 antibody - ChIP Grade (ab12079)
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Western blot - Histone H3 antibody (ab12079)
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Product Name
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Histone H3 antibody - ChIP Grade
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See all Histone H3 - unmodified antibodies (20)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Histone H3 - ChIP Grade
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Immunogen
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Synthetic peptide derived from within residues 100 to the C-terminus of Human Histone H3.
(Peptide available as ab12149.)
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Reacts with
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Hu, Ms, Chk, ChHm, Dog, Dm, Xl
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Predicted to react
(species key)
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At, Ce, Sc, S pom
(Due to sequence homology)
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Tested applications
(see key)
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ChIP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use 2-4µg per 25µg of chromatin. Every new batch of this antibody is tested at Abcam in ChIP.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). Can be blocked with Histone H3 peptide (ab12149).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> H3 >> Unmodified
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Relevance
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.
This antibody is raised using the same immunogen as our popular rabbit polyclonal to Histone H3 - ab1791.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.025% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.78 - 1.10 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 antibody - ChIP Grade images, references, products related to ab12079 and other tools.
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Histone H3 antibody - ChIP Grade images:
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 ChIP - Histone H3 antibody - ChIP Grade (ab12079)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab12079 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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- Histone H3 antibody - ChIP Grade (ab12079)
Histone H3 antibody - ChIP Grade (ab12079) at 0.5 µg/ml + HeLa Cell Histone prep at 5 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L (HRP) (ab6741) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
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Western blot - Histone H3 antibody (ab12079)
All lanes : Histone H3 antibody - ChIP Grade (ab12079) at 1 µg/ml
Lane 1 : Chicken S1:29 Cell lysate at 10 µg
Lane 2 : CHO Cell lysate at 10 µg
Lane 3 : Dog MDCK Cell lysate at 10 µg
Lane 4 : Mouse 3T3 Whole Cell Lysate
Lane 5 : Xenopus embryo Cell lysate at 10 µg
Secondary
Alexa Fluor 680 Rabbit anti-Goat IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
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References for Histone H3 antibody - ChIP Grade (ab12079)
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This product has been used in: (two most recent references)
Tie F et al. CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing. Development 136:3131-41 (2009). WB; Fruit fly (Drosophila melanogaster). PubMed: 19700617
The corresponding rabbit polyclonal ab1791 has been used in:
If you publish research using ab12079 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Histone H3
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