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Product Name
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Histone H3 antibody (HRP)
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See all Histone H3 - unmodified antibodies (19)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Histone H3 (HRP)
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Immunogen
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Synthetic peptide: MARTKQTARKST conjugated to KLH, corresponding to amino acids 1-12 of Human Histone H3
BLAST 'MARTKQTARKST' with  or 
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Reacts with
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Hu, Ms, Rat, Chk
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Predicted to react
(species key)
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Many
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
WB: 1/250 - 1/1000. Predicted molecular weight: 15 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse spleen or mouse brain nuclear lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Histones >> H3 >> Unmodified
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Relevance
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Conjugation notes
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Reductive amination. Ab/HRP ratio=1/2
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Histone H3
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Histone H3 antibody (HRP) - more information
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