Recombinant
RabMAb

Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

Overview

  • Product name
    Anti-Histone H3 (citrulline R2) antibody [EPR17703]
    See all Histone H3 primary antibodies
  • Description
    Rabbit monoclonal [EPR17703] to Histone H3 (citrulline R2)
  • Tested applications
    Suitable for: PepArr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H3 aa 1-100 (citrulline R2). The exact sequence is proprietary. Also SwissProt: P84243, Q71DI3, Q16695, Q6NXT2.
    Database link: P68431

  • Positive control
    • MCF7 cells transfected with PADI4; NIH/3T3 cells transfected with PADI4
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR17703
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
PepArr Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

  • Relevance
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- modifications of histones, also called histone code, and nucleosome remodeling. P68431 and Q71DI3 are expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Q16695 is expressed in tesicular cells. Q6NXT2 is specifically expressed in the seminiferous tubules of testis and this Hominid-specific H3.5/H3F3C preferentially colocalizes with euchromatin, and it is associated with actively transcribed genes. P84243 is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Post-translational modification. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Cit Histone H3 antibody
    • Citrullated antibody
    • citrullin antibody
    • Citrullinated Histone H3 antibody
    • citrulline antibody
    • H3 3 like sequence MH921 antibody
    • H3 3A antibody
    • H3 histone antibody
    • H3 histone family member E pseudogene antibody
    • H3F3 antibody
    • HIST3H3 antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3 Citrullated antibody
    • Histone H3.1 antibody
    • Histone H3.1t antibody
    • Histone H3.2 antibody
    • Histone H3.3 antibody
    • Histone H3.3C antibody
    • Histone H3.5 antibody
    see all

Images

  • All batches of ab176843 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
     
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
     
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • All lanes : Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843) at 1/500 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) cells transfected with vector control
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cells transfected with PADI4

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 15 kDa
    Observed band size : 15 kDa


    Exposure time : 30 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843) at 1/500 dilution

    Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cells transfected with vector control
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cells transfected with PADI4

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 15 kDa
    Observed band size : 15 kDa


    Exposure time : 30 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:
  • Neeli I & Radic M Current Challenges and Limitations in Antibody-Based Detection of Citrullinated Histones. Front Immunol 7:528 (2016). WB ; Human . Read more (PubMed: 27933065) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60 cell line)
Gel Running Conditions
Non-reduced Denaturing (4-20% gradient gel)
Loading amount
40 µg
Treatment
Differentiated in 1.25% DMSO for 5 days, treated with 4uM calcium ionophore A23187 for 15 mins at 37C.
Specification
HL60 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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