Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade (ab5103)

Publishing research using ab5103? Please let us know so that we can cite the reference in this datasheet.

ab5103 has been referenced in 79 publications.

  • Kamioka Y  et al. Intravital Förster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli. Cancer Sci 108:226-235 (2017). IHC-P ; Mouse . PubMed: 27960041
  • Thomas DC  et al. Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity. J Exp Med 214:1111-1128 (2017). ICC/IF . PubMed: 28351984
  • Fan L  et al. PADI4 Epigenetically Suppresses p21 Transcription and Inhibits Cell Apoptosis in Fibroblast-like Synoviocytes from Rheumatoid Arthritis Patients. Int J Biol Sci 13:358-366 (2017). WB ; Human . PubMed: 28367100
  • Mor-Vaknin N  et al. DEK-targeting DNA aptamers as therapeutics for inflammatory arthritis. Nat Commun 8:14252 (2017). IHC (PFA fixed) ; Mouse . PubMed: 28165452
  • Wang L  et al. Resistance of hypervirulent Klebsiella pneumoniae to both intracellular and extracellular killing of neutrophils. PLoS One 12:e0173638 (2017). ICC/IF ; Human . PubMed: 28282434
  • Stoikou M  et al. Gestational Diabetes Mellitus Is Associated with Altered Neutrophil Activity. Front Immunol 8:702 (2017). IHC ; Human . PubMed: 28659928
  • van der Linden M  et al. Differential Signalling and Kinetics of Neutrophil Extracellular Trap Release Revealed by Quantitative Live Imaging. Sci Rep 7:6529 (2017). ICC/IF ; Human . PubMed: 28747804
  • Kenny EF  et al. Diverse stimuli engage different neutrophil extracellular trap pathways. Elife 6:N/A (2017). WB . PubMed: 28574339
  • Leal AC  et al. Tumor-Derived Exosomes Induce the Formation of Neutrophil Extracellular Traps: Implications For The Establishment of Cancer-Associated Thrombosis. Sci Rep 7:6438 (2017). PubMed: 28743887
  • Kim JK  et al. Increased Neutrophil Extracellular Trap Formation in Uremia Is Associated with Chronic Inflammation and Prevalent Coronary Artery Disease. J Immunol Res 2017:8415179 (2017). WB ; Human . PubMed: 28523279
  • Khan SA  et al. GnRH Stimulates Peptidylarginine Deiminase Catalyzed Histone Citrullination in Gonadotrope Cells. Mol Endocrinol 30:1081-1091 (2016). PubMed: 27603413
  • Katkar GD  et al. NETosis and lack of DNase activity are key factors in Echis carinatus venom-induced tissue destruction. Nat Commun 7:11361 (2016). PubMed: 27093631
  • Val S  et al. Proteomic Characterization of Middle Ear Fluid Confirms Neutrophil Extracellular Traps as a Predominant Innate Immune Response in Chronic Otitis Media. PLoS One 11:e0152865 (2016). IF . PubMed: 27078692
  • Konig MF & Andrade F A Critical Reappraisal of Neutrophil Extracellular Traps and NETosis Mimics Based on Differential Requirements for Protein Citrullination. Front Immunol 7:461 (2016). PubMed: 27867381
  • Arelaki S  et al. Gradient Infiltration of Neutrophil Extracellular Traps in Colon Cancer and Evidence for Their Involvement in Tumour Growth. PLoS One 11:e0154484 (2016). PubMed: 27136460
  • Homa J  et al. Conservative Mechanisms of Extracellular Trap Formation by Annelida Eisenia andrei: Serine Protease Activity Requirement. PLoS One 11:e0159031 (2016). IHC-Fr . PubMed: 27416067
  • Li G  et al. Peptidylarginine Deiminase 3 (PAD3) Is Upregulated by Prolactin Stimulation of CID-9 Cells and Expressed in the Lactating Mouse Mammary Gland. PLoS One 11:e0147503 (2016). PubMed: 26799659
  • Wang H  et al. Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis. PLoS One 11:e0148197 (2016). PubMed: 26840412
  • Neeli I & Radic M Current Challenges and Limitations in Antibody-Based Detection of Citrullinated Histones. Front Immunol 7:528 (2016). WB ; Human . PubMed: 27933065
  • Maueröder C  et al. Ménage-à-Trois: The Ratio of Bicarbonate to CO2 and the pH Regulate the Capacity of Neutrophils to Form NETs. Front Immunol 7:583 (2016). ICC/IF ; Human . PubMed: 28018350
  • Biermann MH  et al. Oxidative Burst-Dependent NETosis Is Implicated in the Resolution of Necrosis-Associated Sterile Inflammation. Front Immunol 7:557 (2016). IHC-P ; Mouse . PubMed: 27990145
  • Cortjens B  et al. Pneumovirus-Induced Lung Disease in Mice Is Independent of Neutrophil-Driven Inflammation. PLoS One 11:e0168779 (2016). IHC ; Mouse . PubMed: 28005954
  • Braster Q  et al. Inhibition of NET Release Fails to Reduce Adipose Tissue Inflammation in Mice. PLoS One 11:e0163922 (2016). PubMed: 27701440
  • Skopelja S  et al. The role for neutrophil extracellular traps in cystic fibrosis autoimmunity. JCI Insight 1:e88912 (2016). ICC/IF ; Human . PubMed: 27777975
  • Fine N  et al. Distinct Oral Neutrophil Subsets Define Health and Periodontal Disease States. J Dent Res 95:931-8 (2016). PubMed: 27270666
  • Wake H  et al. Histidine-Rich Glycoprotein Prevents Septic Lethality through Regulation of Immunothrombosis and Inflammation. EBioMedicine 9:180-94 (2016). PubMed: 27333033
  • Bilyy R  et al. Neutrophil Extracellular Traps Form a Barrier between Necrotic and Viable Areas in Acute Abdominal Inflammation. Front Immunol 7:424 (2016). IHC-P ; Human . PubMed: 27777576
  • Chang HH  et al. A molecular signature of preclinical rheumatoid arthritis triggered by dysregulated PTPN22. JCI Insight 1:e90045 (2016). WB ; Human . PubMed: 27777982
  • Karthikeyan K  et al. A Contra Capture Protein Array Platform for Studying Post-translationally Modified (PTM) Auto-antigenomes. Mol Cell Proteomics 15:2324-37 (2016). PubMed: 27141097
  • Sousa-Rocha D  et al. Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors. PLoS One 10:e0139569 (2015). WB . PubMed: 26431537
  • Blachère NE  et al. Inflammatory but not apoptotic death of granulocytes citrullinates fibrinogen. Arthritis Res Ther 17:369 (2015). WB ; Human . PubMed: 26684871
  • Vogel S  et al. Platelet-derived HMGB1 is a critical mediator of thrombosis. J Clin Invest 125:4638-54 (2015). IHC-P, IHC-Fr ; Mouse . PubMed: 26551681
  • Zenaro E  et al. Neutrophils promote Alzheimer's disease-like pathology and cognitive decline via LFA-1 integrin. Nat Med N/A:N/A (2015). PubMed: 26214837
  • Mizurini DM  et al. Salivary Thromboxane A2-Binding Proteins from Triatomine Vectors of Chagas Disease Inhibit Platelet-Mediated Neutrophil Extracellular Traps (NETs) Formation and Arterial Thrombosis. PLoS Negl Trop Dis 9:e0003869 (2015). Mouse . PubMed: 26110417
  • Gavillet M  et al. Flow cytometric assay for direct quantification of neutrophil extracellular traps in blood samples. Am J Hematol 90:1155-8 (2015). Flow Cyt . PubMed: 26347989
  • Lewis HD  et al. Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation. Nat Chem Biol 11:189-91 (2015). PubMed: 25622091
  • Hamaguchi S  et al. Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model. Mediators Inflamm 2015:614518 (2015). WB, Flow Cyt . PubMed: 26273139
  • Rochael NC  et al. Classical ROS-dependent and early/rapid ROS-independent release of Neutrophil Extracellular Traps triggered by Leishmania parasites. Sci Rep 5:18302 (2015). ICC/IF ; Human . PubMed: 26673780
  • Jhunjhunwala S  et al. Neutrophil Responses to Sterile Implant Materials. PLoS One 10:e0137550 (2015). Mouse . PubMed: 26355958
  • Wang S  et al. ATF4 Gene Network Mediates Cellular Response to the Anticancer PAD Inhibitor YW3-56 in Triple-Negative Breast Cancer Cells. Mol Cancer Ther 14:877-88 (2015). WB ; Human . PubMed: 25612620
  • Spengler J  et al. Release of Active Peptidyl Arginine Deiminases by Neutrophils Can Explain Production of Extracellular Citrullinated Autoantigens in Rheumatoid Arthritis Synovial Fluid. Arthritis Rheumatol 67:3135-45 (2015). PubMed: 26245941
  • Loures FV  et al. Recognition of Aspergillus fumigatus hyphae by human plasmacytoid dendritic cells is mediated by dectin-2 and results in formation of extracellular traps. PLoS Pathog 11:e1004643 (2015). PubMed: 25659141
  • Kessinger CW  et al. Statins improve the resolution of established murine venous thrombosis: reductions in thrombus burden and vein wall scarring. PLoS One 10:e0116621 (2015). WB . PubMed: 25680183
  • Yu Y  et al. Celastrol inhibits inflammatory stimuli-induced neutrophil extracellular trap formation. Curr Mol Med 15:401-10 (2015). WB . PubMed: 25941817
  • Boone BA  et al. The receptor for advanced glycation end products (RAGE) enhances autophagy and neutrophil extracellular traps in pancreatic cancer. Cancer Gene Ther 22:326-34 (2015). PubMed: 25908451
  • Zhang D  et al. Neutrophil ageing is regulated by the microbiome. Nature 525:528-32 (2015). IF . PubMed: 26374999
  • Savchenko AS  et al. Neutrophil extracellular traps form predominantly during the organizing stage of human venous thromboembolism development. J Thromb Haemost 12:860-70 (2014). IHC-P ; Human . PubMed: 24674135
  • Waisberg M  et al. Plasmodium falciparum infection induces expression of a mosquito salivary protein (Agaphelin) that targets neutrophil function and inhibits thrombosis without impairing hemostasis. PLoS Pathog 10:e1004338 (2014). ICC/IF ; Human . PubMed: 25211214
  • Branzk N  et al. Neutrophils sense microbe size and selectively release neutrophil extracellular traps in response to large pathogens. Nat Immunol 15:1017-25 (2014). PubMed: 25217981
  • Dwivedi N  et al. Deimination of linker histones links neutrophil extracellular trap release with autoantibodies in systemic autoimmunity. FASEB J 28:2840-51 (2014). WB ; Human . PubMed: 24671707
  • Hirose T  et al. Presence of neutrophil extracellular traps and citrullinated histone H3 in the bloodstream of critically ill patients. PLoS One 9:e111755 (2014). ICC/IF ; Human . PubMed: 25392950
  • Deplus R  et al. Citrullination of DNMT3A by PADI4 regulates its stability and controls DNA methylation. Nucleic Acids Res N/A:N/A (2014). WB . PubMed: 24957603
  • Chen G  et al. Heme-induced neutrophil extracellular traps contribute to the pathogenesis of sickle cell disease. Blood 123:3818-27 (2014). PubMed: 24620350
  • Savchenko AS  et al. VWF-mediated leukocyte recruitment with chromatin decondensation by PAD4 increases myocardial ischemia/reperfusion injury in mice. Blood 123:141-8 (2014). ICC/IF ; Mouse . PubMed: 24200682
  • Darrah E  et al. Erosive rheumatoid arthritis is associated with antibodies that activate PAD4 by increasing calcium sensitivity. Sci Transl Med 5:186ra65 (2013). WB ; Human . PubMed: 23698378
  • Barrientos L  et al. An improved strategy to recover large fragments of functional human neutrophil extracellular traps. Front Immunol 4:166 (2013). PubMed: 23805143
  • Knight JS  et al. Peptidylarginine deiminase inhibition is immunomodulatory and vasculoprotective in murine lupus. J Clin Invest 123:2981-93 (2013). PubMed: 23722903
  • Neeli I & Radic M Opposition between PKC isoforms regulates histone deimination and neutrophil extracellular chromatin release. Front Immunol 4:38 (2013). Human . PubMed: 23430963
  • Martinod K  et al. Neutrophil histone modification by peptidylarginine deiminase 4 is critical for deep vein thrombosis in mice. Proc Natl Acad Sci U S A 110:8674-9 (2013). IHC, ICC/IF ; Mouse . PubMed: 23650392
  • Itakura A & McCarty OJ Pivotal role for the mTOR pathway in the formation of neutrophil extracellular traps via regulation of autophagy. Am J Physiol Cell Physiol 305:C348-54 (2013). PubMed: 23720022
  • Brill A  et al. Neutrophil extracellular traps promote deep vein thrombosis in mice. J Thromb Haemost 10:136-44 (2012). PubMed: 22044575
  • Sharma P  et al. Citrullination of Histone H3 Interferes with HP1-Mediated Transcriptional Repression. PLoS Genet 8:e1002934 (2012). PubMed: 23028349
  • Leshner M  et al. PAD4 mediated histone hypercitrullination induces heterochromatin decondensation and chromatin unfolding to form neutrophil extracellular trap-like structures. Front Immunol 3:307 (2012). WB . PubMed: 23060885
  • Tanikawa C  et al. Regulation of histone modification and chromatin structure by the p53-PADI4 pathway. Nat Commun 3:676 (2012). PubMed: 22334079
  • Thomas GM  et al. Extracellular DNA traps are associated with the pathogenesis of TRALI in humans and mice. Blood 119:6335-43 (2012). IHC-Fr ; Mouse . PubMed: 22596262
  • Wang Y  et al. Anticancer Peptidylarginine Deiminase (PAD) Inhibitors Regulate the Autophagy Flux and the Mammalian Target of Rapamycin Complex 1 Activity. J Biol Chem 287:25941-53 (2012). WB, ChIP . PubMed: 22605338
  • Douda DN  et al. Innate immune collectin surfactant protein d simultaneously binds both neutrophil extracellular traps and carbohydrate ligands and promotes bacterial trapping. J Immunol 187:1856-65 (2011). WB ; Mouse . PubMed: 21724991
  • Slack JL  et al. Protein arginine deiminase 4: a target for an epigenetic cancer therapy. Cell Mol Life Sci 68:709-20 (2011). PubMed: 20706768
  • Causey CP  et al. The development of N-a-(2-carboxyl)benzoyl-N(5)-(2-fluoro-1-iminoethyl)-l-ornithine amide (o-F-amidine) and N-a-(2-carboxyl)benzoyl-N(5)-(2-chloro-1-iminoethyl)-l-ornithine amide (o-Cl-amidine) as second generation protein arginine deiminase (PAD) inhibitors. J Med Chem 54:6919-35 (2011). PubMed: 21882827
  • Li P  et al. Coordination of PAD4 and HDAC2 in the regulation of p53-target gene expression. Oncogene 29:3153-62 (2010). PubMed: 20190809
  • Munks MW  et al. Aluminum adjuvants elicit fibrin-dependent extracellular traps in vivo. Blood 116:5191-9 (2010). PubMed: 20876456
  • Li P  et al. PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps. J Exp Med 207:1853-62 (2010). WB, ICC/IF ; Mouse . PubMed: 20733033
  • Wang Y  et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J Cell Biol 184:205-13 (2009). WB ; Human . PubMed: 19153223
  • Denis H  et al. Functional connection between deimination and deacetylation of histones. Mol Cell Biol 29:4982-93 (2009). WB, ChIP/Chip . PubMed: 19581286
  • Neeli I  et al. Histone deimination as a response to inflammatory stimuli in neutrophils. J Immunol 180:1895-902 (2008). WB, ICC/IF ; Human . PubMed: 18209087
  • Li P  et al. Regulation of p53 target gene expression by peptidylarginine deiminase 4. Mol Cell Biol 28:4745-58 (2008). ChIP, WB ; Human . PubMed: 18505818
  • Miao F  et al. Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17. Mol Endocrinol 20:1562-73 (2006). PubMed: 16497732
  • Mastronardi FG  et al. Increased citrullination of histone H3 in multiple sclerosis brain and animal models of demyelination: a role for tumor necrosis factor-induced peptidylarginine deiminase 4 translocation. J Neurosci 26:11387-96 (2006). PubMed: 17079667
  • Cuthbert GL  et al. Histone deimination antagonizes arginine methylation. Cell 118:545-53 (2004). PubMed: 15339660

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