Recombinant
RabMAb

Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082)

Overview

  • Product name
    Anti-Histone H3 (citrulline R26) antibody [EPR20606]
    See all Histone H3 primary antibodies
  • Description
    Rabbit monoclonal [EPR20606] to Histone H3 (citrulline R26)
  • Tested applications
    Suitable for: WB, Dot blot, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Human Histone H3 aa 1-100 (citrulline R26).
    Database link: P68431

    Synthetic peptide within Human Histone H3 aa 1-100 (citrulline R26). The exact sequence is proprietary.
    Database link: P68431

  • Positive control
    • WB: HEK-293T transfected with GFP-tagged PADI4 (WT) whole cell lysate treated with CaCl2 and Ionomycin; NIH/3T3 transfected with GFP-tagged PADI4 (WT) whole cell lysate treated with CaCl2.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR20606
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab212082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Dot blot 1/1000.
ELISA Use a concentration of 0 - 1 µg/ml.

Target

  • Relevance
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- modifications of histones, also called histone code, and nucleosome remodeling. P68431 and Q71DI3 are expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Q16695 is expressed in tesicular cells. Q6NXT2 is specifically expressed in the seminiferous tubules of testis and this Hominid-specific H3.5/H3F3C preferentially colocalizes with euchromatin, and it is associated with actively transcribed genes. P84243 is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Post-translational modification. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Cit Histone H3 antibody
    • Citrullated antibody
    • citrullin antibody
    • Citrullinated Histone H3 antibody
    • citrulline antibody
    • H3 3 like sequence MH921 antibody
    • H3 3A antibody
    • H3 histone antibody
    • H3 histone family member E pseudogene antibody
    • H3F3 antibody
    • HIST3H3 antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3 Citrullated antibody
    • Histone H3.1 antibody
    • Histone H3.1t antibody
    • Histone H3.2 antibody
    • Histone H3.3 antibody
    • Histone H3.3C antibody
    • Histone H3.5 antibody
    see all

Images

  • Dot blot analysis of Histone H3 (citrulline R26) labeled with ab212082 at 1/1000 dilution.

    Lane 1: Histone H3 (citrulline R26) peptide (aa25-36)

    Lane 2: Histone H3 (Citrulline R17) peptide (aa1-21)

    Lane 3: Histone H3 (Citrulline R8) peptide (aa1-21)

    Lane 4: Histone H3 (Citrulline R2) peptide (aa1-21)

    Lane 5: Histone H3 peptide (aa1-21)

    Lane 6: Histone H3 peptide (aa19-36)

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • All lanes : Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control) then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate
    Lane 2 : HEK-293T transfected with a GFP-tagged PADI4 expression vector then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 15 kDa
    Observed band size : 15 kDa


    Exposure time : 5 seconds

    Histone H3R26 is citrullinated by PADI4, CaCl2 is used as a co-factor according to the literature (PMID: 16567635). Ionomycin is used to improve the modification by PADI4 according to the literature (PMID: 26360112).

    Blocking/Dilution buffer: 5% BSA/TBST.

  • All lanes : Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) transfected with a GFP-tagged empty vector (control) then treated with 10 mM CaCl2 for 2 hours, whole cell lysate
    Lane 2 : NIH/3T3 transfected with a GFP-tagged PADI4 expression vector treated with 10 mM CaCl2 for 2 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 15 kDa
    Observed band size : 15 kDa


    Exposure time : 3 minutes

    Histone H3R26 is citrullinated by PADI4, CaCl2 is used as a co-factor according to the literature (PMID: 16567635). Ionomycin is used to improve the modification by PADI4 according to the literature (PMID: 26360112).

    Blocking/Dilution buffer: 5% BSA/TBST.

  • ELISA using ab212082 labeling Histone H3 (Citrulline R26), Histone H3R26 unmodified, Histone H3 (Citrulline R17), Histone H3 (Citrulline R8), Histone H3 (Citrulline R2) and Histone H3R2/8/17 unmodified at 1 µg/ml. ab212082 was used at a range between 0 -1 µg/ml.

    Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) was used as secondary antibody at 1/2500 dilution.

    ab212082 cross binds H3R8Cit at 14%.

References

ab212082 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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