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Synthetic peptide within Human Histone H3 (di methyl K27). The exact sequence is proprietary.
Our Abpromise guarantee covers the use of ab110472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 17 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
Immunoprecipitation : The nuclear extract derived from 3T3 were immunoprecipitated by 4 µg of ab110472.
Western Blot: Rabbit anti Dimethyl Histone 3 (K9), antibody at 1/1000
Predicted band size : 17 kDa
Note: ~50 kDa and ~22 kDa are the rabbit immunoglobulin heavy and light chain
ab110472, at 5 µg, staining Dimethyl Histone H3 (K27) in 3T3 cells by Immunofluorescence (1).
DAPI shown in (2) and Merged staining in (3).
ab110472 has not yet been referenced specifically in any publications.