Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) (ab115070)


  • Product name
    Histone H3 (di-methyl K27) Quantification Kit (Colorimetric)
    See all Histone H3 (K27 methylation) kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
  • Sensitivity
    2 ng/well
  • Range
    20 ng/well - 5000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (di-methyl K27), a modification enriched at pericentromic heterochromatin, is observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.

    Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) (ab115070) allows the user to specifically measure global di-methylation of histone H3K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applications
    Suitable for: Functional Studiesmore details



    Our Abpromise guarantee covers the use of ab115070 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) images

    • ab115070 used to measure the amount of di-methyl H3K27. Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476).


    References for Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) (ab115070)

    ab115070 has not yet been referenced specifically in any publications.

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