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Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (di-methyl K27), a modification enriched at pericentromic heterochromatin, is observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.
Histone H3 (di-methyl K27) Quantification Kit (Colorimetric) (ab115070) allows the user to specifically measure global di-methylation of histone H3K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Color Developer||1 x 5ml||1 x 10ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115070 used to measure the amount of di-methyl H3K27. Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476).
ab115070 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"