Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)

Overview

  • Product nameHistone H3 (di-methyl K9) Quantification Kit (Colorimetric)See all Histone H3 kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as in heterochromatin formation. Specifically, H3 (di-methyl K9) mediates heterochromatin formation by forming a binding site for HP1.

    Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062) allows the user to specifically measure global di-methylation of histone H3K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsFunctional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab115062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) images

  • Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476) and the amount of dimethyl-H3-K9 was measured using ab115062.

Protocols

References for Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)

ab115062 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab115062.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"