Histone H3 (di-methyl K9) Quantification Kit (Fluorometric) (ab115063)

Overview

  • Product nameHistone H3 (di-methyl K9) Quantification Kit (Fluorometric)
    See all Histone H3 (K9 methylation) kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as in heterochromatin formation. Specifically, H3 (di-methyl K9) mediates heterochromatin formation by forming a binding site for HP1.

    Histone H3 (di-methyl K9) Quantification Kit (Fluorometric) (ab115063) allows the user to specifically measure global di-methylation of histone H3K9 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115063 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (di-methyl K9) Quantification Kit (Fluorometric) images

    • Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476) and the amount of H3K9me2 was measured using ab115063.

    Protocols

    References for Histone H3 (di-methyl K9) Quantification Kit (Fluorometric) (ab115063)

    ab115063 has not yet been referenced specifically in any publications.

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