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Western blot - Histone H3 - HRP antibody (HRP) (ab21054)
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Product Name
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Histone H3 - HRP antibody (HRP)
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Histone H3 - HRP (HRP)
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H3. (Peptide available as ab12149.)
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Reacts with
(species key)
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Hu, Ms, Dm, Sc
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 15.4 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following:
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Yeast S.cerevisiae Whole Cell Lysate
Drosophila Embryo Nuclear Extract
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Cellular localization
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Nuclear
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Research areas
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Tags & Cell Markers >> Epitope Tags >> Conjugates
Chromatin and Nuclear Signaling >> Histones >> H3 >> Unmodified
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Relevance
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Conjugation notes
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This antibody has been conjugated with HRP. The concentration of the original antibody was 0.5 mg/ml, following conjugation the concentration observed will be higher.
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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Batch dependent within range: 0.80 - 1.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 - HRP antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 - HRP antibody (HRP) images, references, products related to ab21054 and other tools.
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Histone H3 - HRP antibody (HRP) images:
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Search PubMed (MEDLINE) for references to Histone H3 - HRP
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Histone H3 - HRP antibody (HRP) - more information
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Histone H3 - HRP antibody (HRP) - other tools:
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Western blot - Histone H3 - HRP antibody (HRP) (ab21054)